葡萄糖转运体4活性抑制对神经母细胞瘤影响

Inhibition of glucose transporter type 4 activity influences neuroblastoma

目的通过体外实验评估GLUT4特异性抑制剂FASENTIN如何调控神经母细胞瘤细胞株SH-SY5Y活力、细胞周期和糖酵解。方法使用抑制剂FASENTIN处理SH-SY5Y细胞72 h,分析细胞活力,QRT-PCR、Western blotting及流式细胞术分析SH-SY5Y细胞中三磷酸腺苷(ATP)和乳酸脱氢酶(LDH)水平。结果FASENTIN处理减弱了肿瘤细胞活力并引起肿瘤细胞坏死而非凋亡(P<0.05)。FASENTIN处理下调了肿瘤细胞内ATP、LDH及糖酵解酶水平(P<0.05)。结论FASENTIN诱导的萄糖转运体4(GLUT4)的活性降低抑制了肿瘤细胞生长,引起细胞周期阻滞并减少糖酵解能力。提示GLUT4可能作为神经母细胞瘤潜在治疗靶标之一。

Objective Evaluated effects of glucose trnsporter type 4(GLUT4)inhibitor FASENTIN on neuro-blastoma cell activity,cell cycle and glycolysis.Methods We treated SH-SY5Y cells with FASENTIN for 72 hours and followed by cell viability assay,QRT-PCR,Western blotting and flow cytometry.We also evaluated adenosine-5-1-triphosphate(ATP)and lactic dehydrogenase(LDH)level in the cells.Results FASENTIN treatment reduced cell viability by inducing cell necrosis but not apoptosis(P<0.05).FASENTIN treatment decreased ATP,LDH and glycolysis level in cells(P<0.05).Conclusion FASENTIN induced GLUT4 activity inhibition suppressed tumor cell growth,induced cell cycle arrest and reduced glycolysis.This study implied that GLUT4 could be a potential therapeutic target for neuroblastoma.