摘要
目的优化山楂、猴头菇功能饮料中总多糖的提取工艺。方法采用苯酚-硫酸显色法测定多糖的含量。以提取物中多糖的含量为评价指标,通过单因素实验初步确定最佳的提取温度、料液比例、提取时间。然后采用响应面实验设计进行提取工艺的优化。结果最佳提取工艺:提取温度为70℃,料液比为1∶25,提取时间为66 min。结论本方法工艺简单、快速、重复性好,可作为山楂、猴头菇功能饮料中总多糖的提取应用。
Objective To optimize the extraction process of total polysaccharides from hawthorn and Hericium erinaceus functional drinks.Methods The content of polysaccharide was determined by phenol sulfuric acid colorimetry.Taking the content of polysaccharide in the extract as the evaluation index,the optimum extraction temperature,material liquid ratio and extraction time were determined by single factor experiment.Then response surface design was used to optimize the extraction process.Results The optimum extraction conditions were as followsthe extraction temperature was 70℃,the solid-liquid ratio was 1∶25,and the extraction time was 66 min.Conclusion The method is simple,rapid and reproducible,and can be used to extract total polysaccharides from hawthorn and Hericium erinaceus functional drinks.
作者
门志雯
崔佰吉
苏琳婷
李松岩
孙海宁
肖雯
欧阳静
沈楠
刘庆波
MEN Zhiwen;CUI Baiji;SU Linting;LI Songyan;SUN Haining;XIAO Wen;OUYANG Jing;SHEN Nan;LIU Qingbo(School of Basic Medicine,Jilin Medical University,Jilin City,Jilin Province,132013;2,Jilin Hospital of Integrated Traditional Chinese and Western Medicine,Jilin City,Jilin Province,132012,China)
出处
《吉林医药学院学报》
2021年第5期345-347,共3页
Journal of Jilin Medical University
基金
吉林省中医药科技项目(2019040)
全国中药特色技术传承人才培训项目(T20184828005).
关键词
猴头菇
山楂
多糖
响应面设计
Hericium erinaceus
Hawthorn
polysaccharide
response surface design
