摘要
目的优化紫姜药材中甲氧基黄酮的提取工艺。方法采用超声提取法,在单因素考察的基础上,结合信息熵-响应面法对乙醇体积分数、超声时间、液料比进行考察,以紫姜中6种主要甲氧基黄酮类成分的含量为评价指标,根据信息熵理论,赋予各指标权重系数,计算综合评分。通过Design-Expert 10.0.7软件分析各因素间的交互作用,得出优化的提取工艺,并对该优化工艺进行验证实验。结果优化的工艺条件为:乙醇体积分数为60%,液料比为12∶1,超声提取2次,每次25 min,甲氧基黄酮类成分的综合评分为1.024,验证实验结果为0.994,各指标成分含量及综合评分RSD值均小于1%。结论优化的提取工艺稳定可行,可为紫姜的进一步研究提供参考。
Objective To optimize the extraction process of methoxylflavones in the rhizome of Kaempferia parviflora.Methods Ultrasonication was used for the extraction.Based on single factor experiment and information entropy-response surface method,three factors including ethanol volume fraction,extraction time and liquid-to-material ratio were investigated.Taking the yields of 6 main methoxylflavones as evaluation indexes,the weight coefficient of each index was calculated according to the information entropy theory,and the comprehensive score was obtained.With Design-Expert 10.0.7 software,the interactions between the 3 factors were analyzed.The optimized extraction condition was obtained and the validation test was performed.Results The optimized extraction condition was as follows:ethanol volume fraction of 60%,liquid to material ratio of 12∶1 and extracted twice,25 minutes each times.The predicted comprehensive score was 1.024 and the verification test result was 0.994 with RSD value was less than 1%.Conclusion The optimized extraction process is stable and feasible,which can provide scientific reference for further research and development of the the rhizome of Kaempferia parviflora.
作者
李格
张静
韩福国
郝艳丽
范雪梅
赵重博
刘清飞
LI Ge;ZHANG Jing;HAN Fuguo;HAO Yanli;FAN Xuemei;ZHAO Chongbo;LIU Qingfei(Shaanxi University of Chinese Medicine,Xianyang 712046,China;School of Pharmaceutical Sciences,Tsinghua University,Beijing 100084,China)
出处
《西北药学杂志》
2021年第4期545-551,共7页
Northwest Pharmaceutical Journal
基金
科技部国际合作重点项目(编号:2016YFE113700)。
关键词
紫姜
响应面法
信息熵理论
综合评分
工艺优化
rhizome of Kaempferia parviflora
response surface method
information entropy theory
comprehensive score
technology optimization