梭梭根际解磷菌的分离及其解有机磷能力

Isolation of Organophosphate-degradation Bacteria from Rhizosphere of Haloxylon ammodendron and Their Organophosphate-degradation Capability

以内蒙古巴丹吉林沙地药用植物梭梭为材料,采用传统分离培养技术,使用有机磷培养基对梭梭根际解磷菌进行分离,基于16S rRNA基因序列分析进行分类鉴定,采用钒钼比色法测定其解有机磷能力,以植酸酶活性表示。从梭梭根际土壤中分离纯化得到8株解有机磷细菌,分属根瘤菌属(Rhizobium)、鞘氨醇单胞菌属(Sphingomonas)、假单胞菌属(Pseudomonas)、肠杆菌属(Enterobacter)、泛菌属(Pantoea)、马西利亚菌属(Massilia)和节杆菌属(Arthrobacter)。变形菌门(Proteobacteria)是梭梭根际极优势类群,占总数的87.5%。其中,菌株Y29和B333解有机磷能力最强,植酸酶活分别为24.21,20.43μg·mL^(-1),是2株极具开发潜力的解磷菌。本文揭示了梭梭根际可培养解有机磷细菌的类群组成,为认识和利用荒漠解磷菌提供了理论依据。

Traditional isolation and culture technology and organic phosphorus media were used to isolate organophosphate-degradation bacteria in the rhizosphere of medicinal plant Haloxylon ammodendron from the sandland of Badainjaran in Inner Mongolia.A preliminary identification was based on the 16S rRNA gene.Vanadium-ammonium-molybdate was used to measure the organophosphate-degradation capabilities,and the organophosphate-degradation cability was indicated by phytase activity.The results showed that a total of 8 organophosphate-degradation bacteria were isolated,which belonged to Rhizobium,Sphingomona,Pseudomonas,Enterobacter,Pantoea,Massilia and Arthrobacter,respectively.The Extremely dominant class was Proteobacteria with ratio of 87.5%.The strains Y29 and B333 had the strongest ganophosphate-degradation ability,and the phytase activity was 24.21,20.43μg·mL^(-1),respectively,which were of great potential applicationvalue.This study preliminarily revealed the composition of cultivable organophosphate-degradation bacteria from rhizosphere of Haloxylon ammodendron,which provides a basis for understanding and using desert organophosphate-degradation bacteria for applications.