超高效液相色谱法同时测定颈舒颗粒中4种皂苷类成分含量

Simultaneous Determinations of Four Saponins in Jingshu Granules by UPLC

目的建立同时测定颈舒颗粒中三七皂苷R_(1)、人参皂苷Rb_(1)、人参皂苷Rg_(1)、人参皂苷Re含量的超高效液相色谱法。方法色谱柱为Waters BEH C_(18)柱(50 mm×2.1 mm,1.7μm),流动相为乙腈-水(梯度洗脱),流速为0.6 m L/min,检测波长为203 nm,柱温为30℃,进样量为1μL。结果三七皂苷Rb_(1)、人参皂苷Rb_(1)、人参皂苷Rg_(1)、人参皂苷Re在各自含量范围内与峰面积线性关系良好(r≥0.9991,n=7);精密度、稳定性、重复性试验的RSD均小于2%;平均回收率分别为101.23%,101.36%,100.29%,99.31%,RSD分别为1.25%,1.32%,0.89%,1.34%(n=6)。结论该方法操作快速简便,专属性强,重复性好,准确度较高,可用于颈舒颗粒中三七皂苷Rb_(1)、人参皂苷Rg_(1)、人参皂苷Rb_(1)、人参皂苷Re 4种成分的含量测定。

Objective To establish an ultra high-performance liquid chromatography(UPLC)method for the simultaneous determination of notoginsenoside R_(1),ginsenoside Rb_(1),ginsenoside Rg_(1)and ginsenoside Re in Jingshu Granules.Methods The chromatographic column was Waters BEH C_(18)column(50 mm×2.1 mm,1.7μm),the mobile phase was acetonitrile-water with gradient elution,the flow rate was 0.6 m L/min,the detection wavelength was 203 nm,the column temperature was 30℃,and the injection volume was 1μL.Results Notoginsenoside R_(1),ginsenoside Rb_(1),ginsenoside Rg_(1),and ginsenoside Re showed a good linear relationship with the peak area in their respective mass ranges(r≥0.9991,n=7).The RSDs of precision,stability and repeatability tests were less than 2%.The average recoveries of notoginsenoside R_(1),ginsenoside Rb_(1),ginsenoside Rg_(1),and ginsenoside Re were 101.23%,101.36%,100.29%and99.31%with RSDs of 1.25%,1.32%,0.89%and 1.34%(n=6),respectively.Conclusion The method is rapid,simple,specific,reproducible,and accurate,which can be used for the content determination of notoginsenoside R_(1),ginsenoside Rb_(1),ginsenoside Rg_(1),ginsenoside Re in Jingshu Granules.