超高效液相色谱-串联质谱法检测中药饮片中8种黄色素类染色剂

Determination of Eight Yellow Pigments Adulterated in Chinese Herbal Pieces by UPLC-MS/MS

目的建立同时测定中药饮片中8种黄色素类染色剂含量的超高效液相色谱-串联质谱法。方法样品采用乙醇-水(7∶3,V/V)超声提取。色谱柱为Thermo Acclaim^(TM)RSLC 120 C_(18)柱(100 mm×2.1 mm,3.0μm),流动相为乙腈-0.02 mol/L乙酸铵溶液(梯度洗脱),流速为0.2 m L/min,柱温为30℃,进样量为5μL;电离方式为正离子电喷雾离子源(ESI+),选择多反应监测模式进行定量分析。结果8种黄色素类染色剂在各自线性范围内均与峰面积线性关系良好(r=0.9979~0.9995);平均回收率为89.83%~99.52%,RSD为2.14%~3.67%(n=6)。结论该方法简单快速、灵敏准确,可用于中药饮片中黄色素类染色剂含量的检测。

Objective To establish an ultra high-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method for the simultaneous determination of eight yellow pigments adulterated in Chinese herbal pieces.Methods The samples were extracted by ethanol-water(7∶3,V/V)with ultrasonic.The chromatographic column was Thermo Acclaim^(TM)RSLC 120 C_(18)column(100 mm×2.1 mm,3.0μm),the mobile phase was acetonitrile-0.02 mol/L ammonium acetate solution with gradient elution,the flow rate was0.2 m L/min,the column temperature was 30℃,and the injection volume was 5μL.The ionization mode was positive ion electrospray ionization source(ESI+),and multiple reaction monitoring mode(MRM)was selected for quantitative analysis.Results The eight yellow pigments showed a good linear relationship with peak area in their respective linear range(r=0.9979-0.9995).The average recovery of eight yellow pigments was in the range of 89.83%-99.52%with RSDs of 2.14%-3.67%(n=6).Conclusion The method is simple,rapid,sensitive and accurate,which can be used for the determination of yellow pigments adulterated in Chinese herbal pieces.