上海市2例输入性卵形疟误诊病例的实验室诊断分析

Laboratory diagnosis of two misdiagnosed imported Plasmodium ovale malaria cases in Shanghai

上海市疾病预防控制中心(简称上海市疾控)采用显微镜检查、疟疾快速诊断检测(RDT)和疟疾核酸检测[NP-2002法、多重PCR法和荧光定量PCR(qPCR)法]对2例虫种分型存在争议的输入性疟疾患者的血样进行实验室诊断与分析。结果显示,上海市A区疾病预防控制中心(简称A区疾控)对患者1的初检结果为镜检查见恶性疟原虫,RDT检测结果为恶性疟阳性,未作核酸检测;上海市疾控对患者1的复核结果是镜检查见卵形疟原虫,RDT检测结果为恶性疟阳性,核酸检测结果为卵形疟原虫阳性。B区疾控分别采用镜检、RDT和qPCR法对患者2进行初检,结果均为间日疟阳性;上海市疾控对患者2的复核结果为镜检查见卵形疟原虫,RDT结果为阴性,核酸检测结果为卵形疟阳性。

Laboratory diagnosis was performed using microscopic examination,rapid malaria diagnostic test(RDT)and malaria nucleic acid test(NP-2002 method,multiplex PCR method and qPCR method)on two blood samples of imported malaria patients,to which there was controversy on the Plasmodium species.The initial examination of case 1 in a district-level center for disease control and prevention(CDC)in region A found P.falciparum(Pf)by microscopy,and Pf by RDT,whereas nucleic acid test was not done.The examination result was rechecked by Shanghai Municipal Center for Disease Control and Prevention(SCDC),presenting confirmation of P.ovale(Po)by microscopy,Pf by RDT,and Po by nucleic acid detection.The initial examination results of case 2 using microscopy,RDT,and nucleic acid detection in the district B CDC all revealed P.vivax(Pv).The recheck results of SCDC were Po by microscopy,negative finding by RDT,and Po by nucleic acid detection.