植物来源神经导管负载EMSCs移植修复大鼠坐骨神经损伤

Transplantation of plant-derived nerve conduit loaded with EMSCs for rat sciatic nerve repairment

目的:基于天然植物管状茎秆构建一种新型神经导管,搭载鼻黏膜外胚层间充质干细胞(EMSCs)移植修复SD大鼠坐骨神经损伤。方法:利用节节草茎秆和纤维蛋白制备神经导管;通过免疫荧光技术标记EMSCs的标志蛋白Nestin观察EMSCs在体外构建的神经导管上的生长情况来评价导管的生物相容性;用SD大鼠制备坐骨神经损伤模型,移植负载EMSCs的神经导管修复SD大鼠坐骨神经损伤,通过坐骨神经指数、背根神经节内荧光标记阳性感觉神经元数量、腓肠肌纤维的横截面面积和神经损伤/导管移植区域组织内神经丝蛋白200(NF-200)和髓鞘碱性蛋白(MBP)相对表达量评价移植EMSCs/神经导管对神经的修复作用。结果:构建的神经导管与EMSCs具有良好的生物相容性;移植EMSCs/神经导管的实验动物的坐骨神经指数、背根神经节内荧光标记阳性感觉神经元数量、腓肠肌纤维的横截面面积均优于其他实验组;神经损伤/导管移植区域组织内NF-200和MBP的相对表达量高于其他实验组。结论:移植负载EMSCs的植物源性神经导管可有效促进SD大鼠坐骨神经损伤的神经再生和后肢运动功能恢复。

Objective: To construct a novel nerve conduit based on natural plant tubular stalks,and then transplant this conduit with nasal ectomesenchymal stem cells (EMSCs) to repair sciatic nerve injury in rats. Methods: Tubular stalks of the equisetum ramosissimum were used as the original material to construct nerve conduit;Immunofluorescence technique was used to label nestin in EMSCs so that the biocompatibility between the constructed nerve conduits and EMSCs in vitro were evaluated by observing EMSCs growth. Afterward,SD rats were used to establish sciatic nerve injury model. The nerve conduit loaded with EMSCs was transplanted in SD rats to repair sciatic nerve injury. Through sciatic nerve index,the number of fluorescence labelled sensory neurons in dorsal root ganglia,cross-sectional area of gastrocnemius muscle fibers and relative expression of neurofilament protein 200 (NF-200) and myelin basic protein (MBP) of injured area,the condition of sciatic nerve injury repair can be observed. Results: EMSCs had good biocompatibility with the constructed nerve conduit;Compared with other treatments,the nerve conduit/EMSCs showed better outcomes in the functional and morphological repair of the injured sciatic nerves. Sciatic nerve index,the number of fluorescence labelled sensory neurons in dorsal root ganglia,cross-sectional area of gastrocnemius muscle fibers and relative expression of NF-200 and MBP of injured area transplanted with nerve conduit/EMSCs were higher than those of other experimental groups. Conclusion: The transplanted plant-derived nerve conduit/EMSCs can effectively promote the regeneration of the injured sciatic nerve and the recovery of hindlimb motor function in SD rats.