单细胞技术与干细胞疗法在心脏疾病研究中的应用

The Application of Single Cell Technology and Stem Cell-Based Therapy in Cardiac Disease

细胞是生命的基本单位,而异质性则是细胞的固有属性之一。单细胞测序技术的出现,为细胞间异质性的探索、新的细胞群的发现、发育轨迹重建的研究提供了便利。目前,单细胞测序技术已在包括心肌梗死、心肌肥大、心力衰竭在内的研究中有了一定的成果。该文综述了单细胞测序的流程,包括样品制备、文库构建和测序分析,回顾了其在心脏疾病中的应用,并讨论了基于干细胞的疗法在临床中的应用前景。

Cells are the basic unit of life,and heterogeneity is one of the inherent properties of cells.The emergence of single-cell RNA sequencing technology facilitates the study of intercellular heterogeneity,the discovery of new cell populations,and the reconstruction of developmental trajectories.Currently,driven by single-cell RNA sequencing technology,there have been many new advances in the field of myocardial infarction,hypertrophy,and heart failure.This review summarizes the process of single-cell RNA sequencing,including sample preparation,library construction and sequencing analysis,reviews its application in heart diseases,and discusses the clinical prospect of stem cell-based therapies.