摘要
细胞是生命的基本单位,而异质性则是细胞的固有属性之一。单细胞测序技术的出现,为细胞间异质性的探索、新的细胞群的发现、发育轨迹重建的研究提供了便利。目前,单细胞测序技术已在包括心肌梗死、心肌肥大、心力衰竭在内的研究中有了一定的成果。该文综述了单细胞测序的流程,包括样品制备、文库构建和测序分析,回顾了其在心脏疾病中的应用,并讨论了基于干细胞的疗法在临床中的应用前景。
Cells are the basic unit of life,and heterogeneity is one of the inherent properties of cells.The emergence of single-cell RNA sequencing technology facilitates the study of intercellular heterogeneity,the discovery of new cell populations,and the reconstruction of developmental trajectories.Currently,driven by single-cell RNA sequencing technology,there have been many new advances in the field of myocardial infarction,hypertrophy,and heart failure.This review summarizes the process of single-cell RNA sequencing,including sample preparation,library construction and sequencing analysis,reviews its application in heart diseases,and discusses the clinical prospect of stem cell-based therapies.
作者
凤琦
王利
FENG Qi;WANG Li(Fuwai Hospital,Chinese Academy of Medical Sciences,Beijing 100037,China;State Key Laboratory of Cardiovascular Disease,National Center for Cardiovascular Diseases,Beijing 102308,China)
出处
《中国细胞生物学学报》
CSCD
2021年第7期1362-1371,共10页
Chinese Journal of Cell Biology
基金
国家自然科学基金(批准号:82025004、82088101)资助的课题。
关键词
单细胞测序技术
干细胞疗法
心脏疾病
single-cell RNA sequencing
stem cell-based therapy
cardiac disease