结球甘蓝无蜡粉亮叶性状dCAPS标记引物优化

Optimization of dCAPS molecular marking for bright leaf traits of cabbage without wax powder

研究以野生型结球甘蓝98-1030和无蜡粉亮叶突变型98-1030作为父母本材料,杂交得到F_(1)代,将父母本作全基因组测序,根据所得区间内非同义SNP位点,共设计20对dCAPS引物,命名为DC01~DC20,利用亲本及F1代筛选引物。PCR扩增结果表明,有10对dCAPS引物扩增出条带,限制性内切酶鉴定表明,其中有8对引物PCR产物可酶切产生特异性片段。

In this study,wild-type cabbage 98-1030 and the mutant 98-1030 were used as parental materials to obtain F_(1) generation by hyridization.Whole genome sequencing was performed on the parents.A total of 20 pairs of dCAPS primers were designed according to the non-synonymous SNP sites within the obtained range,named as DC01-DC20,and the parents and F1 generation were used for screening.PCR amplification results showed that 10 pairs of dCAPS primers could amplify bands,and restriction enzyme identification showed that PCR products of eight pairs of primers could produce specific fragments by enzyme digestion.