不同检测方法在丙型病毒性肝炎诊断中的应用

Evaluation of Clinical Application of Different Methods in Hepatitis C Diagnosis

目的探讨不同实验室检测方法在丙型病毒性肝炎诊断中的应用及效果评价。方法收集2018年1月—2020年1月临床诊断为丙型肝炎的患者100例,酶联免疫法、磁微粒化学发光法、胶体金法检测丙肝抗体、实时荧光定量聚合酶链反应(polymerase chain reaction,PCR)方法检测丙型肝炎病毒核糖核酸(hepatitis C virus-ribonucleic acid,HCV-RNA),评估不同检测方法在丙型病毒性肝炎临床诊断中的应用价值。结果磁微粒化学发光法阳性率为99.00%,酶联免疫法阳性率为98.00%,略高于胶体金的94.00%,但三种检测方法阳性率之间的差异无显著统计学意义(P>0.05);实时荧光定量PCR法的阳性率为53.00%,可用于检测已确诊的丙型肝炎病毒(hepatitis virus C,HCV)感染患者的病毒载量。结论综合检测HCV抗体和HCV-RNA载量可以明确诊断丙型肝炎感染和判断病毒复制情况。

Objective To explore the application and effect evaluation of different laboratory detection methods in the diagnosis of hepatitis C.Methods 100 patients with hepatitis C clinically diagnosed from January 2018 to January 2020 were collected.Hepatitis C antibody was detected by enzyme-linked immunosorbent assay,magnetic particle chemiluminescence,colloidal gold method,and hepatitis C virus ribonucleic acid(HCV-RNA)was detected by real-time fluorescence quantitative polymerase chain reaction(PCR),the value of different detection methods in the clinical diagnosis of hepatitis C were evaluated.Results The positive rates of magnetic particle chemiluminescence and enzyme-linked immunosorbent assay were 99.00%and 98.00%,slightly higher than 94.00%of colloidal gold,but there was no significant difference among the three methods(P>0.05);the positive rate of real-time fluorescence quantitative PCR was 53.00%,which can be used to detect the viral load of patients with confirmed hepatitis C virus(HCV)infection.Conclusion Comprehensive detection of HCV antibody and HCV-RNA load can clearly diagnose hepatitis C infection and judge virus replication.