性成熟中华绒螯蟹脑、性腺的性别差异调控机制研究

Sexual differentiation regulation mechanism in the brain and gonad of Eriocheir sinensis during reproduction

本研究采用比较转录组法探讨性成熟期中华绒螯蟹(Eriocheirsinensis)雌雄个体间脑、性腺的关键差异调控通路及繁殖调控的关键基因。结果表明,性成熟雌雄蟹其脑、性腺组织具有性别差异调控模式。脑内差异表达通路主要涉及信号转导、性激素调控及环境适应应答,性腺内关键差异调控通路主要涉及激素调控、氨基酸代谢调控等。脑内繁殖调控关键基因主要涉及发育及内稳态调控等,如Ⅲ型纤连蛋白域结合蛋白3a (FNDC3A)、反式甲酸2 (INF2)、神经胶质蛋白(NRG)、假苷酸合酶7同系物(PUS7)、小泛素相关修饰子3 (SUMO3)、超氧化物歧化酶(SOD)等,性腺内关键调控基因主要涉及能源物质代谢、性细胞发育调控等,如甾醇调控原件结合蛋白1 (SREBF1)、卵泡刺激激素受体(FSHR)、表皮生长因子受体(EGFR)、丝/苏氨酸激酶4 (TSSK4)、胰岛素样生长因子2 mRNA结合蛋白3 (IGF2BP3)、磷脂酰肌醇3激酶调节亚基2 (PIK3R2)、胞质型多聚腺苷酸化原件结合蛋白(CPEB)等。本研究结果表明,性成熟河蟹雌雄个体间的脑、性腺具有差异调控模式,本转录组获得的差异调控通路及基因将为进一步开展河蟹繁殖调控研究提供丰富的理论信息。

Eriocheir sinensis is an important catadromous aquatic species in Yangtze River, China, and is one of the leading species for special new variety breeding. E. sinensis is popular both in domestic and foreign markets for its unique flavor. The breeding industry for this fish has developed prosperously;however, precocity is still a hindrance on development. Undoubtedly, researching the physiological regulation mechanism of gonadal development would benefit the E. sinensis breeding industry. However, there are few studies on the mechanism of sexual differentiation regulation in crabs during reproduction. In this study, we performed comparative transcriptome analysis to explore the key differential regulatory pathways in the brains and gonads of male and female E. sinensis during reproduction, as well as the common regulatory genes in these two tissues for reproductive regulation. The results indicated a substantial sexual differentiation regulation mode in the brains and gonads of sexually mature males and females of E. sinensis. The differential regulatory pathways in the brain involved signal transduction, sexual hormone regulation, and adaptive response to environment. The key regulatory pathways in the gonad involved hormone regulation and amino acid metabolism regulation. The key genes relevant to reproduction regulation in the brain were primarily those involved in development and homeostasis regulation, such as FNDC3 A, INF2, NRG, PUS7, SUMO3, and SOD. Key regulatory genes in the gonad were primarily those involved in energy substance metabolism and sexual cell development, such as SREBF1, FSHR, EGFR, TSSK4, IGF2 BP3, PIK3 R2, and CPEB. These results provide theoretical information for further exploration into sexual differentiation regulation in the reproduction of E. sinensis, theoretical references for gender control techniques, and theoretical basis for promoting the development of the E. sinensis breeding industry.