微孔多聚糖止血粉成分及体外生物降解分析

Ingredient and Biodegradation in Vitro Analysis of Microporous Polysaccharide Hemostatic Powder

通过体外生物降解微孔多聚糖止血粉,探究止血粉的主要成分,阐明其生物降解的动态变化规律,为临床使用止血粉提供代谢依据。通过三氟乙酸体外降解止血粉,通过离子色谱法测定产生的单糖物质,从而确定止血粉的组成。利用离子色谱法和分光光度法测定止血粉在37℃、pH=6.9和Ca 2+存在的条件下,经α-淀粉酶作用不同时间后的葡萄糖、麦芽糖和淀粉等含量,研究止血粉生物降解特性。结果表明,止血粉酸降解后得到的单糖皆为葡萄糖,止血粉中葡萄糖的含量为97.8%±2.6%;且止血粉在37℃α-淀粉酶作用下,淀粉含量随着降解时间延长逐步减少,从即时的95.6%至48h的8.0%,1h内降解速率最大,约降解了62.9%。降解产物中除有葡萄糖单糖、麦芽糖双糖外,还有不同聚合度的寡糖;离子色谱图中乳糖为α淀粉酶中物质,在降解的48h乳糖含量保持不变。100×10-6止血粉在37℃α-淀粉酶作用下,葡萄糖的含量从即时的0至48h的4.004×10-6,麦芽糖的含量从即时的0至48h的44.692×10-6,1h内麦芽糖生成速率最大,增加了36.509×10-6。因此,微孔多聚糖止血粉具有良好的生物降解性,是一种良好的安全的止血材料。

By in vitro biodegradation of microporous polysaccharide hemostatic powder,to explore the main ingredient of hemostatic powder,to clarify the dynamics of its biodegradation,and to provide metabolic basis for clinical use of hemostatic powder.Firstly,the hemostatic powder was degraded by trifluoroacetic acid in vitro,and the produced monosaccharide substance was determined by ion chromatography to determine the ingredient of the hemostatic powder.Then,the contents of glucose,maltose and starch in hemostatic powder treated withα-amylase at 37℃,pH=6.9 and Ca 2+were determined by ion chromatography and spectrophotometry,and the biodegradation characteristics of hemostatic powder were studied.The in vitro trifluoroacetic acid degradation test of hemostatic powder showed that all the monosaccharides obtained after acid degradation were glucose,the content of glucose in hemostatic powder was(97.8±2.6)%;and the starch content of hemostatic powder under the action ofα-amylase at 37℃decreased gradually with the extension of degradation time,and the degradation rate was the highest from 95.6%in real time to 8.0%in 48 hours,which was about 62.9%.In addition to glucose monosaccharides and maltose disaccharides,there were oligosaccharides with different degrees of polymerization in the degradation products,and the lactose content was the same as that inα-amylase in ion chromatography,and the lactose content remained unchanged after 48 hours of degradation.Under the action ofα-amylase at 37℃,the glucose content of 100 ppm hemostatic powder ranged from immediate 0 ppm to 48h maltose content from 4.004 ppm,and the maltose content ranges from 0 ppm at once to 44.692 ppm at 48h.The maltose production rate was the highest in one hour,which increased by 36.509 ppm.Microporous polysaccharide hemostatic powder has good biodegradability and is a good and safe hemostatic material.