淮南次生生物煤层气形成途径研究

Study on Formation Pathway of Secondary Biological Coalbed Methane in Huainan Coal Seam

利用淮南新庄孜煤矿采集的煤样、矿井水样开展淮南煤层生物产气的可行性研究及产气途径分析。实验样品采自A1煤层63301切眼,采深786 m煤样的工业组分包括煤样水分含量1.2%、挥发分29.23%、固定碳含量82.72%。实验对照组为矿井水样、煤样、缓冲试剂(N-TES)、ORP指示剂、微生物生长所需的维生素、微量元素、矿物质混合。实验组在对照组的基础上分别添加产乙酸菌抑制剂盐酸万古霉素、二氧化碳吸收液(反应器中内置NaOH溶液,与液体培养基隔离)。经过56 d实验,整个反应过程pH值在7~7.9,适合微生物生存,TOC浓度保持在750~950 mg/L,底物相对充足,最终产气率对照组(0.36μL/g煤)>NaOH吸收液组(0.28μL/g煤)>盐酸万古霉素组(0.26μL/g煤),该结果表明淮南煤层可生物产气,同时存在乙酸氧化途径和CO 2/H 2还原途径,以乙酸途径为主。

Using the coal and mine water samples taken from Xinzhuangzi Coal Mine,Huainan,feasibility studies and analysis of gas production pathways for biological gas production were studied.Collected from A1 coal seam 63301 cut holes,mining depth 786 meters,the industrial components of the sample including the moisture content,volatile content and fixed carbon content were 1.2%,29.23%and 82.72%,respectively.The control group included mine water and coal sample,buffer reagent(N-TES),ORP indicator,vitamins,trace elements and minerals needed for microbial growth were mixed.On the basis of the control group,vancomycin hydrochloride,acetogenic bacterium inhibitor,and carbon dioxide absorption liquid(Sodium hydroxide solution in the reactor,isolated from the liquid medium)were added to the experimental group.The experiment lasted for 56 days,the pH value of the whole reaction process was between 7 and 7.9,suitable for the survival of microorganisms.The TOC concentration was kept between 750 mg/L and 950 mg/L,and the substrate was relatively sufficient.The results showed that the gas production rate of the control group(0.36μL/g coal)>NaOH absorption liquid group(0.28μL/g coal)>the vancomycin hydrochloride group(0.26μL/g coal).The study showed that Huainan coal seam has the potential of biogas production,and there are two ways to produce gas:acetic acid pathway and CO 2/H 2 pathway,while the main pathway was acetic acid pathway.