一株植物内生真菌漆斑菌次级代谢产物的研究

Secondary metabolites of the endophytic fungus Myrothecium sp.from Pinellia ternata

从一株植物内生漆斑菌属Myrothecium sp.真菌固体发酵物的乙酸乙酯提取物中分离得到12个化合物。采用硅胶、Sephadex LH-20和制备高效液相等柱色谱技术进行分离纯化,通过理化性质研究及波谱学手段对单体化合物进行结构鉴定,分别鉴定为3'-羟基疣孢菌素A(1)、疣孢菌素A(2)、乙酰基疣孢菌素L(3)、疣孢菌素J(4)、疣孢菌素K(5)、漆斑菌素A(6)、漆斑菌素D(7)、漆斑菌素H(8)、漆斑菌素J(9)、verrol 4-acetate(10)、(3S,3aS,6α,6aR)-dihydrosporothrioride(11)和4,6-二羟基-1(3H)-异苯并呋喃(12)。其中,化合物1、5、9~12为首次从漆斑菌属真菌中分离得到,化合物2、3、4、7和8对白色念珠菌有较强的抑制作用,其最低抑菌浓度MIC_(50)分别是0.318、0.218、0.047、0.569和0.558μg/mL。

Using a series of purification methods including silica gel,Sephadex LH-20 and preparative high performance liquid chromatography,secondary metabolites of Myrothecium sp.were purified from the ethyl acetate extract of the solid fermentation product.Based on structure characterization and investigation on the physical and chemical properties a,twelve monomeric compounds were identified as 3'-hydroxyverrucarin A(1),verrucarin A(2),verrucarin L acetate(3),verrucarin J(4),verrucarin K(5),roridin A(6),roridin D(7),roridin H(8),roridin J(9),verrol 4-acetate(10),(3S,3aS,6α,6aR)-dihydrosporothrioride(11)and 4,6-dihydroxy-1(3H)-isobenzofuranone(12).Compounds 1,5 and 9-12 were isolated from Myrothecium sp.for the first time.Compounds 2,3,4,7 and 8 exhibited strong inhibitory effects on Candida albicans,with a minimal inhibitory concentration(MIC_(50))of 0.318,0.218,0.047,0.569 and 0.558μg/mL,respectively.