摘要
An enzyme-responsive polysaccharide supramolecular targeted nanoassembly was successfully constructed by the host-guest complexation of positively charged mono-(6-(tetraethylenepentamine)-6-deoxy)-β-cyclodextrin(TEPA-CD)with adamantane-grafted hyaluronic acid(HA-ADA).Possessing a series of positively charged polyamine chains,the obtained polysaccharide nanoassembly could serve as a biocompatible plasmid DNA(p DNA)container.More interestingly,the p DNA could be released from the nanoassembly through the enzymatic degradation of HA skeleton,which realized the controlled p DNA binding and release.Besides,the polysaccharide nanoassembly exhibited lower cytotoxicity than the commercial transfection reagents 25 k Da b PEI(PEI_(25 k)),accompanied by similar gene delivery effect.We believe that this work might present a convenient method for targeted,controlled gene delivery.
基金
the Programs of Higher-level Talents of Inner Mongolia Agricultural University(No.NDGCC2016-21)
the Program for Young Talents of Science and Technology in Universities of Inner Mongolia Autonomous Region(No.NJYT-19-B26)
the Grassland Talents of Inner Mongolia Autonomous Region(No.DC2000000745)
the Inner Mongolia Autonomous Region Natural Science Fund Project(No.2017BS0206)for financial support。
