摘要
Numerous nanocarriers have been currently developed for intracellular delivery.The potential cytotoxicity of these very small inorganic nanocarriers has raised great consideration.Thus,it becomes of utmost importance to conduct the intracellular trace of nanocarriers.Among many analytical techniques,surface enhanced Raman scattering(SERS)method is one of the current state-of-the-art techniques for cell visualization and trace.In this work,a novel stellate porous silica based gene delivery system has been designed for SERS trace purpose.A stellate porous silica nanoparticle modified with many small Au nanoparticles is designed to replace common metallic SERS tags.The results show that the designed system not only could deliver si RNA into cells for therapy,but also could realize SERS trace with high sensitivity and non-invasive features.The constructed delivery system has considerable potential to trace the dynamic gene delivery in living cells.
基金
financially supported by the Australian Research Council(ARC)Discovery Projects(Nos.DP140104062 and DP160104866)
Fundamental Research Funds for the Central Universities(Nos.FRF-TP-19-017B1,2302015-06500017,FRF-BR-19-003B,FRF-BD-20-14A)
National Natural Science Foundation of China(No.21501009)。
