肝癌患者血清乙肝病毒e抗原与肝细胞HBVcccDNA水平的相关性研究

Association between serum hepatitis B virus e antigen and hepatocyte HBV cccDNA levels in patients with liver cancer

目的探讨肝细胞癌(HCC)患者血清乙肝病毒e抗原(HBeAg)与肝细胞乙型肝炎病毒(HBV)cccDNA水平的相关性研究。方法选取2015年1月至2018年1月间抚顺市传染病医院收治的接受肝癌切除术的62例原发性肝细胞癌患者。术后收集HCC患者癌组织及癌旁组织,检测HBV感染标志物、血清HBV DNA以及组织cccDNA。结果根据HBeAg检测结果,将62例HCC患者分为A组:HBeAg(+)/HBeAb(-)14例,B组:HBeAg(+)/HBeAb(+)10例,C组:HBeAg(-)/HBeAb(+)38例。三组患者性别、年龄比较,差异均有统计学意义(均P<0.05);三组肿瘤最大直径、肿瘤个数比较,差异无统计学意义(P>0.05);A组血清HBV DNA、癌旁组织HBV DNA及cccDNA水平高于C组,差异均有统计学意义(均P<0.05),A组癌旁组织cccDNA高于B组,差异有统计学意义(P<0.05),B组癌组织HBV DNA高于C组,差异有统计学意义(P<0.05)。HCC患者血清HBeAg与癌旁组织cccDNA和HBV DNA具有相关性(P<0.05)。HCC患者血清HBeAg与癌组织cccDNA和HBV DNA无相关性(P>0.05)。血清HBV DNA仅在TNMⅠ期及Ⅲ期患者与癌旁组织cccDNA和HBV DNA具有相关性(P<0.05)。结论血清HBeAg与癌旁组织HBV DNA和cccDNA具有相关性,且不受TNM分期影响。

Objective To observe the association between serum hepatitis B virus e antigen( HBe Ag) and hepatocyte HBV ccc DNA levels in patients with hepatocellular carcinoma( HCC). Methods Sixty-two patients with HCC who underwent hepatic resection were selected at Fushun Infectious Disease Hospital from January 2015 to January 2018. Cancer tissues and precancerous tissues were collected from these patients after surgery. HBV infection markers,serum HBV DNA and ccc DNA were detected. Results According to the HBe Ag test results,62 HCC patients were divided into group A( 14 HBe Ag-positive and HBe Ab-negative patients),group B( 10 HBe Ag-positive and HBe Ab-positive patients) and group C( 38 HBe Ag-negative and HBe Ab-positive patients). There were statistically significant differences in gender and age among the three group( all P < 0. 05). There was no statistically significant difference in the largest tumor diameter and the number of tumors among the three groups( P > 0. 05);The levels of serum HBV DNA,HBV DNA in precancerous tissues and ccc DNA levels were higher in group A than in group C( P <0. 05). The ccc DNA levels in precancerous tissues were higher in group A than in group B( P < 0. 05).The HBV DNA of cancer tissue in group B was higher than in group C( P < 0. 05). Serum HBe Ag was associated with ccc DNA and HBV DNA in precancerous tissues in HCC patients( all P < 0. 05). There was no correlation between HBe Ab and ccc DNA and HBV DNA in cancerous tissues in HCC patients( P >0. 05). Serum HBV DNA was only associated with ccc DNA and HBV DNA in precancerous tissues in patients with TNM stage Ⅰ and Ⅲ liver cancer( all P < 0. 05). Conclusion Serum HBe Ag is associated with HBV DNA and ccc DNA in precancerous tissues,and is not affected by TNM staging.