miR-138、miR-149表达与非肌层浸润性膀胱癌患者临床病理特征及预后的关系

Relationship between the expression levelsof miR-138 and miR-149 in non-muscle invasive bladder cancer and clinicopathological characteristics and prognosis

目的:探讨非肌层浸润性膀胱癌(NMIBC)组织中微小RNA(miR)-138和miR-149的表达与NMIBC患者临床病理特征和预后的关系。方法:收集2013年1月至2015年2月在青海省西宁市第一人民医院行手术治疗的49例NMIBC患者的组织样本及其癌旁组织,采用实时荧光定量PCR(RT-qPCR)法检测组织中miR-138和miR-149的表达水平,并分析miR-138和miR-149表达水平与NMIBC患者临床病理特征的关系。以miR-138和miR-149表达水平的中位数为界,将NMIBC患者分为高表达组和低表达组,随访5年,采用Kaplan-Meier生存曲线分析miR-138和miR-149表达水平与NMIBC患者预后的关系。结果:与癌旁组织相比,miR-138、miR-149在癌组织中的表达显著降低(P<0.05)。单因素分析结果显示,有淋巴结转移和远处转移的NMIBC患者癌组织中miR-138和miR-149的表达水平显著降低(P<0.05)。Kaplan-Meier生存曲线分析结果显示,miR-138低表达组、miR-149低表达组的5年生存率分别低于miR-138高表达组和miR-149高表达组,术后生存时间分别短于miR-138高表达组和miR-149高表达组(均P<0.05)。结论:NMIBC患者癌组织中miR-138和miR-149均呈低表达,且在淋巴结转移和远处转移的患者中表达更低,其与生存预后有一定关联,可为NMIBC患者的转移风险预测和预后提供参考。

Objective:To investigate the relationship between the expression levelsof miR-138 and miR-149 and the clinicopathological characteristics and prognosis of patients with non-muscle invasive bladder cancer(NMIBC).Methods:Thecancertissue samples and corresponding paracancer tissues from 49 patients with NMIBC who were treated by surgery in our hospital from January 2013 to February 2015 were selected.The expression levels of miR-138 and miR-149 in NMIBC tissues were detected by real-time fluorescence quantitative PCR(RT-qPCR).The relationship between the expression levels of miR-138 and miR-149 and the clinicopathological characteristics of patients with NMIBC were analyzed.Based on the median expression level of miR-138 and miR-149 as the boundary,patients with NMIBC were divided into high expression group and low expression group,followed up for 5 years,and Kaplan-Meier survival curve was used to analyze the relationship between the expression levels of miR-138 and miR-149 and the prognosis of patients with NMIBC.Results:The expression of miR-138 and miR-149 in cancer tissues were decreased compared with the corresponding paracancer tissues(P<0.05).Univariate analysis showed that the expression levels of miR-138 and miR-149 in the cancer tissues of NMIBC patients with lymph node metastasis and distant metastasiss were lower than those of NMIBC patients without lymph node metastasis and distant metastasis(P<0.05).The results of Kaplan-Meier survival curve analysis showed that the 5-year survival rate of the miR-138 low-expression group and the miR-149 low-expression group were significantly lower than those of the miR-138 high-expression group and the miR-149 high-expression group,and the postoperative survival time was shorter than that of the miR-138 high-expression group and the miR-149 high-expression group(P<0.05).Conclusion:Both miR-138 and miR-149 are lowexpressed in the cancer tissues of patients with NMIBC,and the expression level is lower in NMIBC patients with lymph node metastasis and distant metastasis,which is correlated with survival prognosis to a certain extent,and it can provide important reference value for metastasis risk prediction and prognosis of patients with NMIBC.