基于网络药理学的山豆根抗癌机制研究

Research on Anticancer Mechanism of Sophora tonkinensis Gagnep Based on Network Pharmacology

[目的]运用网络药理学反向分子对接的原理,对山豆根抑癌作用机制进行预测。[方法]筛选山豆根化学成分25个,通过PharmMapper数据库查找相关靶点,NCBI数据库进行靶点的校正,利用DAVID数据库筛选药物的KEGG通路,运用cytoscape建立山豆根的“药材-化学成分-靶点-作用通路-药理作用-临床应用”多层次作用网络图。[结果]山豆根的25个化学成分可调控CTBP2、CDKN1B、ABL1等51个靶点,得到相关通路25条,涉及癌症、炎症、肝病、心血管病等疾病,其中10条通路与癌症相关。[结论]通过网络药理学的分析方法,预测了山豆根的抗癌作用机制,为深入研究山豆根的药理作用提供方向。

[Objective]To predict the antitumor mechanism of Sophora tonkinensis by using the principle of reverse molecular docking in network pharmacology.[Method]25 chemical constituents of Sophora tonkinensis were screened,and the related targets were searched through the pharmapper database,the targets were adjusted by NCBI database,the KEGG pathway of drugs was screened by DAVID database,and the multi-level action network diagram of“medicinal materials-chemical composition-target-action pathway-pharmacological action-clinical application”was established by using Cytoscape.[Result]The 25 chemical components of Sophora tonkinensis could regulate 51 targets such as CTBP2,CDKN1B and ABL1,and 25 related pathways were obtained,involving cancer,inflammation,liver disease,cardiovascular disease and other diseases.Ten of them are related to cancer.[Conclusion]The anti-cancer mechanism of Sophora tonkinensis is predicted by network pharmacology analysis method,which provides direction for further study on pharmacological action of Sophora tonkinensis.