克罗恩病患者海马区静息态功能磁共振成像改变的观察

Resting-state fMRI study of hippocampus in patients with Crohn′s disease

对2018年2月至2019年6月在上海市静安区中心医院经临床确诊的15例缓解期克罗恩病患者(克罗恩病组)及与其相匹配的26例健康者(对照组),行静息状态下海马区功能磁共振成像检查,计算低频振幅值,观察两组间低频振幅存在差异的脑区;以左侧海马为种子点,计算该区域与全脑的功能连接特征。与对照组相比,克罗恩病组左侧海马区(体素32)的低频振幅降低[整体错误校正(FWE校正),簇水平,P<0.05],左内侧额上回、辅助运动区及左侧前部扣带回(体素分别为126、126、37)低频振幅增高(FWE校正,簇水平,P<0.05);以左侧海马区为种子点进行全脑功能连接分析显示,克罗恩病组与左侧颞上回、左内侧额上回、左侧岛盖部额下回及右侧辅助运动区的功能连接增强(FWE较正,簇水平,P<0.05);病程与差异脑区低频振幅/功能连接值无显著相关性(P>0.05)。提示克罗恩病患者左侧海马区自发性活动及功能连接存在改变。

Fifteen patients with Crohn′s disease(CD)in remission diagnosed at Shanghai Jing′an District Central Hospital from February 2018 to June 2019,and 26 matched healthy subjects were recruited.All participants underwent resting-state functional magnetic resonance imaging(fMRI)scans of hippocampus.The amplitude of low-frequency fluctuations(ALFF)was calculated to determine differences in the brain.Left hippocampus was selected as seeds for functional connectivity(FC)analysis,and the results were compared between two groups.The relationship between disease duration and ALFF/FC values in abnormal regions were analyzed with Pearson correlation.Compared with the controls,the ALFF of the left hippocampus(voxel size 32)of CD patients decreased[family-wise error correstion(FWE correction),cluster level P<0.05],and the ALFF of the left medial superior frontal gyrus(voxel size 126),left supplementary motor area(voxel size 126)and left anterior cingulate gyrus increased(voxel size 37)(FWE corrected,cluster level P<0.05).Using the left hippocampus as the seed point for the whole brain functional connectivity analysis,CD patients showed increased FC strength with the left superior temporal gyrus,left medial superior frontal gyrus,left inferior frontal gyrus(opercular part),and right supplementary motor area(FEW corrected,cluster level P<0.05).Correlation analysis did not show a significantly differences between ALFF/FC value of altered brain areas and the disease duration.It suggests that there are changes in spontaneous activities and functional connectivity in the left hippocampus in patients with CD.