金纳米颗粒-微气泡复合材料及其体外成像和光热性能研究

Controlled assembly of gold nanoparticles on microbubbles for ultrasound imaging and photothermal therapy in vitro

目的:构建两种结构的金纳米颗粒-微气泡复合材料,既能作为超声成像造影剂,又可以利用金纳米颗粒的表面等离激元效应进行光热转换。方法:一种是将金纳米颗粒包裹在微气泡气体内核中的包金微气泡(gold encapsulated microbubble,AuMB),另一种是通过共价键结合的方式将金纳米颗粒装载到微气泡表面的载金微气泡(polyethyleneimine-gold loaded microbubble,PEI-AuMB),对这两类金纳米颗粒-微气泡复合材料进行理化性质的表征,通过体外超声成像评估其超声造影能力,通过体外光热实验评估其光热转换能力。结果:两种复合结构的微气泡均显示出比空白微气泡(microbubble,MB)更好的超声造影能力,其中PEI-AuMB在超声造影增强的持续时间上有更好的表现;两种复合结构的微气泡也显示出比单纯金纳米颗粒更优异的光热转换能力,AuMB有更好的光热治疗潜力。结论:金纳米颗粒-微气泡复合材料是具有潜力的诊疗一体化载体系统。

Objective:Two gold nanoparticle-microbubble composites with different structures were constructed,which can be used as ultrasound imaging contrast agents and for photothermal conversion using the surface plasmonic excitonic effect of gold nanoparticles. Methods:One is gold encapsulated microbubble(AuMB)with gold nanoparticles encapsulated in a microbubble gas core,and the other is polyethyleneimine-gold loaded microbubble(PEI-AuMB)with gold nanoparticles loaded onto the microbubble surface by covalent bonding. These gold nanoparticle-microbubble composites were characterized for their physicochemical properties,and their ultrasound contrast capabilities were evaluated by in vitro ultrasound imaging. Their photothermal conversion ability was evaluated by in vitro photothermal experiments. Results:Both composite microbubbles showed better ultrasonography ability than blank microbubble(MB),among which PEI-AuMB showed better performance in the duration of ultrasonography enhancement;both composite microbubbles also showed superior photothermal conversion ability than gold nanoparticles alone,and AuMB had better photothermal therapeutic potential. Conclusion:All these results demonstrates that gold nanoparticle-microbubble composites are promising carrier systems for therapeutic integration.