血清miR-219水平与急性缺血性脑卒中后轻度认知功能障碍的关系

Relationship between serum miR-219 level and mild cognitive impairment after acute ischemic stroke

目的检测miR-219在急性缺血性脑卒中(AIS)患者血清中的水平,探讨miR-219与AIS后轻度认知功能障碍的关系。方法选择2018年11月至2019年10月在该院神经内科住院的AIS患者185例为研究对象,按照认知功能评价分为轻度认知功能障碍组和无认知功能障碍组。另选取体检健康者170例作为对照组,采用实时荧光定量PCR检测各组研究对象血清中miR-219水平,用二分类Logistic回归分析影响AIS患者发生轻度认知功能障碍的危险因素,用受试者工作特征(ROC)曲线分析血清miR-219水平对AIS患者发生轻度认知功能障碍的预测价值。结果185例AIS患者中,有轻度认知功能障碍95例,无认知功能障碍90例。轻度认知功能障碍组糖尿病比例明显高于无认知功能障碍组,差异有统计学意义(P<0.05);对照组、无认知功能障碍组、轻度认知功能障碍组AIS患者血清miR-219水平依次升高,两两比较,差异有统计学意义(P<0.05);二分类Logistic回归分析显示,糖尿病、血清miR-219水平高是AIS患者发生轻度认知功能障碍的独立危险因素;ROC曲线分析结果显示,血清miR-219水平预测AIS患者发生轻度认知功能障碍的ROC曲线下面积为0.818,最佳截断值为1.34。结论miR-219在AIS患者血清中明显高表达,是影响AIS后轻度认知功能障碍发生的危险因素,对其有一定预测价值。

Objective To detect the level of miR-219 in the serum of patients with acute ischemic stroke(AIS),and to explore the relationship between miR-219 and mild cognitive impairment after AIS.Methods A total of 185 AIS patients admitted to the department of neurological in Dalian Municipal Third People′s Hospital from November 2018 to October 2019 were selected and divided into mild cognitive impairment group and non-cognitive impairment group according to the cognitive function evaluation.Other 170 healthy persons undergoing the physical examination were selected as control group.The level of serum miR-219 in all subjects was detected by qRT-PCR.The dualistic Logistic method was used to analyze the risk factors affecting the AIS patients to develop mild cognitive impairment,and the receiver operating characteristic(ROC)curve was used to analyze the predictive value of serum miR-219 level for mild cognitive impairment occurrence in AIS patients.Results There were 95 cases with mild cognitive impairment and 90 cases with non-cognitive impairment among 185 AIS patients.The proportion of diabetes mellitus in mild cognitive impairment group was significantly higher than that in non-cognitive impairment group(P<0.05).The level of serum miR-219 in control group,non-cognitive impairment group and mild cognitive impairment group increased in turn(P<0.05).The dualistic Logistic regression analysis showed that diabetes mellitus and high serum miR-219 level were the independent risk factors for mild cognitive impairment occurrence in AIS patients.The ROC curve analysis results showed that the area under ROC curve of serum miR-219 for predicting the mild cognitive impairment occurrence in AIS patients was 0.818,and the best cutoff value was 1.34.Conclusion miR-219 highly expresses in the serum of AIS patients,which is a risk factor affecting mild cognitive impairment occurrence,and has certain diagnostic value for mild cognitive impairment occurrence in AIS patients.