芽孢杆菌LM基于全基因组的分类鉴定及抑菌原理的研究

Classification and Identification of Bacillus LM Based on Whole Genome and Study on Its Antibacterial Principle

对一株抗黑曲霉活性的芽孢杆菌进行了基因组和超高效液相色谱质谱(UPLC-Q-TOF-MS)分析研究。结合形态特征、16S rRNA序列和gyrA基因聚类分析,初步鉴定菌株为解淀粉芽孢杆菌(Bacillus amyloliquefaciens);全基因组分析表明菌株基因组大小为4000885 bp,GC含量为46.24%;antiSMASH分析表明基因组中含11个次级代谢产物基因簇,大多编码抗菌物质;UPLC-Q-TOF-MS分析发现10个化合物的分子量与antiSMASH预测的3个基因簇编码的化合物分子量相同,为表面活性素(surfactin)、伊枯草菌素(iturin)和芬荠素(fengycin)等脂肽类抗生素同系物,推测解淀粉芽孢杆菌的抗黑曲霉活性与这3类物质有关。

Bacillus LM,which inhibits Aspergillus niger,was analyzed with genomics and UPLC-Q-TOF-MS.Combined with morphological characteristics,16S rRNA sequence and gyrA gene cluster analysis,the strain was primarily identified as Bacillus amyloliquefaciens.The analysis of whole genome showed that the genome size of the strain was 4000885 bp and GC content was 46.24%.The analysis of antiSMASH demonstrated that there were 11 secondary metabolite gene clusters and most of them encoded antibacterial substances.UPLC-Q-TOF-MS analysis revealed that the molecular weights of 10 chemical compounds were consistent with those by 3 predicted gene clusters via antiSMASH,which were lipopeptide antibiotics-like surfactin,iturin and fengycin.It could be speculated that the B.amyloliquefaciens activity of suppressing A.niger was associated with those 3 substances.