基于生物信息学分析卵巢癌免疫细胞浸润程度及其与预后的关系

Correlation analysis of immune cell infiltration pattern and prognosis in ovarian cancer based on bioinformatics methods

目的探讨免疫细胞浸润程度与卵巢癌生存预后的关系。方法通过生物信息学方法,从GEO数据库下载卵巢癌患者的组织芯片测序数据和临床分期、病理分级、生存状态、生存时间等信息。通过ssGSEA基因集的各项免疫评分,将卵巢癌患者分为高分组和低分组。通过生存曲线比较滤泡辅助性T细胞(Tfh)高、低评分组的生存预后情况。通过Cibersort软件计算肿瘤组织中各类免疫细胞浸润的比例,并进行相关性分析。结果Tfh评分与预后生存有关(P<0.05)。Tfh高分组的浆细胞、活化的NK细胞、M1型巨噬细胞浸润程度明显高于低分组(P<0.05),而M2型巨噬细胞的浸润程度则明显低于低分组(P<0.05)。结论卵巢癌中存在不同程度的免疫细胞浸润,Tfh浸润可能是影响卵巢癌患者预后的因素。

Objective To explore the correlation between immune cell infiltration and prognosis of ovarian cancer.Methods Downloaded the microarray sequencing data,clinical stage,tumor classification,survival status,survival time and other information of ovarian cancer patients from the GEO database by using bioinformatics methods.By using the immune score of ssGSEA gene set,divided the ovarian cancer patients into high-score group and low-score group.The survival curve was used to compare the survival prognosis between high and low T cells follicular helper(Tfh)-score group.Calculated the proportion of various types of immune cells infiltration in tumor tissues through Cibersort software,and perform correlation analysis.Results Tfh score was related to prognostic survival(P<0.05).The degree of infiltration of plasma cells,activated NK cells,and M1 macrophages in the high Tfh-score group was significantly higher than that in the low Tfh-score group(P<0.05),while the infiltration degree of M2 macrophages was significantly lower than that of the low Tfh-score group(P<0.05).Conclusion There are varying degrees of immune cell infiltration in ovarian cancer,and Tfh infiltration may be a factor affecting the prognosis of patients with ovarian cancer.