2020年北疆部分地区猪伪狂犬病毒gE蛋白抗体ELISA检测与分析

Detection and Analysis of Antibody to gE Protein of PRV in Some Areas of Northern Xinjiang in 2020 by ELISA

为了解2020年1—12月北疆部分地区猪伪狂犬病野毒株(PRV-gE)感染情况,采用间接酶联免疫吸附试验(ELISA)法对从北疆4个地区采集的657份猪血样进行PRV-gE蛋白抗体水平检测。结果显示,4个地区PRV-gE抗体阳性率为29.83%(196/657),乌苏地区与其他地区的抗体水平存在一定差异(P<0.05);各猪场中A场的阳性检出率最高,为83.78%(62/74);D场和H场的阳性检出率最低,为0.00%;不同类别猪群中,保育猪的PRV-gE蛋白抗体阳性率最高,为46.77%(29/62);哺乳仔猪抗体水平与其他猪群均存在一定差异(P<0.05)。试验表明,各个猪群均存在野毒感染情况,各养猪场之间、不同生长阶段感染情况不一。该试验可为北疆地区猪场PR防控与净化提供参考。

In order to understand the infection of pig pseudorabies wild strain(PRV-g E)in some areas of northern Xinjiang from January to December in 2020.410 pigs blood samples were collected from 4 regions of northern Xinjiang and tested by the indirect enzyme-linked immunosorption test(ELISA)method.The results showed that the positive rates of PRV-g E antibody in 4 regions were 29.83%(196/657)and different antibody levels in Wusu area and other regions(P<0.05).The highest positive detection rates were farm A in each pig farm,83.78%(62/74).Farm D and farm H’s positive rate was 0.00%.Among the different groups of nursery pigs,the highest PRV-g E protein antibody positive rates were 46.77%(29/62).Nursing piglets’antibody levels were different from other pig populations(P<0.05).The experiment showed that there was wild poison infection in each pig group,and the infection situation varied between the pig farms and at different growth stages.It provided reference for PR prevention,control and purification of pig farms in northern of Xinjiang.