转基因抗虫水稻‘HD2’不同生长发育阶段cry2A^(*)基因表达量

Expression Amount of cry2A^(*) Gene at Different Growth and Development Stages of Transgenic Pest-resistant Rice‘HD2’

本研究旨在探究转cry2A^(*)基因抗虫水稻‘HD2-1’等独立系在田间生长发育过程中cry2A^(*)基因表达量及糙米Bt蛋白含量(BPC)。利用qRT-PCR方法和酶联免疫吸附法(ELISA)检测水稻‘HD2-1’等独立系田间不同生长发育阶段组织器官及糙米的cry2A^(*)基因表达量。结果显示,不同水稻独立系cry2A^(*)基因表达量及糙米BPC明显不同,叶片、茎鞘及幼穗cry2A^(*)基因表达量在不同生长发育阶段间往往呈正相关,cry2A^(*)基因mRNA表达量和对应的BPC呈极显著的正相关,糙米BPC和各生长发育阶段各器官cry2A^(*)基因表达量存在正相关趋势。水稻‘HD2’cry2A^(*)基因表达量不同生长发育阶段间及其和糙米BPC间呈正相关。cry2A^(*)基因转录表达越高,BPC越高。

The purpose is to investigate the expressing amount of cry2A^(*) gene and brown rice Bt protein content(BPC) in transgenic cry2A^(*) Gene Resistant Rice‘HD2-1’and other independent lines during the growth and development stages. qRT-PCR and enzyme-linked immunosorbent assay(ELISA) were used to detect the expression amount of cry2A^(*)gene of organs at different growth and development stages and brown rice BPC. The results showed that there was a significant difference among rice independent lines in expression amount of cry2A^(*) gene and BPC of brown rice. There were often positive correlation of the expression amount of cry2A^(*) gene of leaf blade, sheath-culm and young panicle among different growth and development stages.The mRNA expression of cry2A^(*) gene was positively correlated with the corresponding BPC. And there was a positive correlation trend between BPC of brown rice and expression amount of cry2A^(*) gene of each organ at each stage. In expression amount of cry2A^(*) gene of rice‘HD2’, there were positive relations among different growth and development stages and between the ones and BPC of brown rice. The higher the cry2A^(*) gene transcribed, the higher the BPC was.