布康唑与其他四种唑类药物对外阴阴道假丝酵母菌病耐药菌株药物敏感性差异研究

Study on the sensitivity difference of buconazole and other four azole drugs to candida vulvovaginal candidiasis resistant strains

目的研究布康唑、氟康唑、伊曲康唑、咪康唑和克霉唑对耐药性假丝酵母菌株的药物敏感性差异。方法参照美国临床实验室标准化协会(CLSI)推荐的M27-A3方案的微量液基稀释法,测量并比较五种唑类药物抗120株耐药性白假丝酵母菌和8株非白假丝酵母菌的最低抑菌浓度(MIC)。结果 (1)布康唑对白假丝酵母菌、光滑假丝酵母菌和克柔假丝酵母菌的24 h MIC值低于其他四种唑类抗真菌药物;(2)伊曲康唑对耐克霉唑白假丝酵母菌的MIC值(1.82μg/ml)高于耐药界定值(1μg/ml);(3)耐氟康唑组、耐咪康唑组和耐伊曲康唑组中,克霉唑对白假丝酵母菌的MIC值(0.56μg/ml、0.39μg/ml和0.52μg/ml)远低于敏感界定值(2μg/ml),伊曲康唑的MIC值(2.44μg/ml、2.11μg/ml和2.23μg/ml)远高于耐药界定值(1μg/ml)。结论布康唑对耐药性假丝酵母菌的药物敏感性优于其他四种唑类药物。

Objective To study the differences of drug sensitivity of buconazole, fluconazole, itraconazole, miconazole and clotrizole to drug-resistant candida strains. Methods The minimum inhibitory concentration(MIC) of five azole drugs against 120 strains of drug-resistant candida albicans and 8 strains of non-candida albicans were measured and compared according to the microliquid-based dilution method of M27-A3 regimen recommended by the American Association for Clinical Laboratory Standardization(CLSI). Results(1) The 24 h MIC of buconazole was lower than that of other four kinds of antifungal drugs.(2) The MIC value of itraconazole against candida albicans niketriazole(1.82 μg/ml) was higher than the defined value of resistance(1 μg/ml).(3) In the fluconazole resistant group, miconazole resistant group and itraconazole resistant group, the MIC values of cloxazole(0.56 μg/ml, 0.39 μg/ml and 0.52 μg/ml) were far lower than the sensitive value(2 μg/ml), and the MIC values of itraconazole(2.44 μg/ml, 2.11 μg/ml and 2.23 μg/ml) were far higher than the resistant value(1 μg/ml). Conclusions The drug sensitivity of buconazole to drug resistant candida is better than the other four azole drugs.