摘要
目的:观察大黄-赤芍中药颗粒溶解物对肝衰竭大鼠模型肝组织Gab1与Akt表达的调控作用。方法:Wistar雄性大鼠45只,随机分为空白组、模型组、大黄-赤芍组,每组15只。造模前48 h开始灌胃给药,一直延续至造模后48 h。模型对照组给予等量蒸馏水。腹腔注射D氨基半乳糖600 mg/kg+LPS 20μg/kg,复制肝衰竭动物模型,造模成功后48 h收集各组大鼠肝组织样本,采用荧光定量PCR及Western Blot检测肝组织Gab1、Akt基因及蛋白表达情况。结果:与模型对照组比较,大黄-赤芍组大鼠肝组织Gab1、Akt mRNA及蛋白表达量增高,差异具有统计学意义(P<0.05)。结论:大黄-赤芍中药颗粒溶解物可能是通过增强Gab1-Akt信号传导途径,在一定程度上能够改善肝衰竭大鼠肝组织微循环状态。
Objective:To observe the regulation of Dahuang and Chishao on the expression of Gab1 and Akt in liver tissue of rats with liver failure.Methods:Forty five male Wistar rats were randomly divided into blank group,model control group and herb treatment group,with 15 rats in each group.The animal model of liver failure was established by intraperitoneal injection of D galactosamine 600 mg/kg+LPS 20μg/kg.The liver tissue samples were collected 48 hours after successful modeling.The expression of Gab1 and Akt in liver tissue were detected by Western blot and fluorescence quantitative PCR.Results:The protein and mRNA expressions of Gab1 and Akt in liver tissue of Dahuang and Chishao treatment group were significantly higher than those of model control group(P<0.05).Conclusion:Dahuang and Chishao can improve the microcirculation of liver tissue in rats with liver failure by enhancing the signal transduction pathway of Gab1Akt.
作者
王明刚
WANG Ming-gang(The First Affiliated Hospital of Guangxi University of traditional Chinese medicine,Nanning Guangxi,530023,China)
出处
《中西医结合肝病杂志》
CAS
2021年第8期718-720,725,共4页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
基金
广西高校中青年教师基础能力提升项目(No.KY2016YB208)
广西高校重点学科经费支持(No.2017XK04)
广西自然科学基金创新研究团队项目(No.2018GXNSFGA281002)
广西自然科学基金(No.2017GXNSFBA198200,No.2018GXNSFAA281047)。
