HPLC法测不同中药中蛇床子素的含量

Determination of osthol in different traditional Chinese medicines by HPLC

目的建立不同中药中蛇床子素的含量测定方法。方法采用Agilent ZORBAX SBC18色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-体积分数0.05%磷酸溶液(体积比70:30),流速为1.0 mL·min^(-1),检测波长为322 nm,柱温30℃。结果蛇床子素在质量浓度为0.100~1.90 g·L^(-1)内与峰面积呈良好的线性关系。精密度、重复性及稳定性试验的RSD分别为0.36%、0.28%、0.45%,均符合要求。高、中、低平均加样回收率分别为98.35%、100.78%、101.4%。蛇床子、菟丝子、独活、白芷、白前等中药材中蛇床子素的含量分别为7.74、0.0230、7.42、0.0419、4.43 mg·g^(-1)。结论建立的高效液相色谱法方法简便,准确,快速,重复性好,专属性好,可应用于不同中药中蛇床子素的含量测定。

Objective To establish a method for the determination of osthole in different traditional Chinese medicines.Methods Agilent Zorbax SB-C18 column(250 mm×4.6 mm,5μm)was used.The mobile phase was methanol-0.05%phosphoric acid solution(V:V=70:30).The flow rate was 1.0 mL·min^(-1).The detection wavelength was 322 nm and the column temperature was 30℃.Results The linear range of osthole was 0.100~1.90 g·L^(-1).The RSD of precision,repeatability and stability were 0.36%,0.28%and 0.45%respectively,which met the requirements.The average recoveries of high,medium and low were 98.4%,100.8%and 101.4%,respectively.The contents of osthol in Cnidium monnieri,Cuscuta chinensis,Dodder,Angelica dahurica and Radix Angelicae dahuricae were 7.74,0.0230,7.42,0.0419 and 4.43 mg·g^(-1),respectively.Conclusion The method is simple,accurate,rapid,reproducible and specific,and can be used for the determination of osthole in different traditional Chinese medicines.