茶藨子木层孔菌化学成分及其抗肿瘤活性

Chemical constituents from Phellinus ribis and their antitumor activities

目的研究茶藨子木层孔菌Phellinus ribis的化学成分及其抗肿瘤活性。方法茶藨子木层孔菌甲醇提取物采用硅胶、Sephadex LH-20、半制备型HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用MTT法评价其抗肿瘤活性。结果从中分离得到4个化合物,分别鉴定为inoscavin C(1)、phellifuropyranone A(2)、phelligridin D(3)、inoscavin A(4),均能够在一定程度上抑制HepG2细胞的增殖,对HepG2细胞的抑制作用在5~160μmol/L浓度范围内,呈剂量依赖性增长。结论所有化合物均为首次从该真菌中分离得到,并均具有抗肿瘤活性。

AIM To study the chemical constituents from Phellinus ribis and their antitumor activities.METHODS The methanol extract from P.ribis was isolated and purified by silica,Sephadex LH-20,and semipreparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.Their antitumor activities were evaluated by MTT method.RESULTS Four compounds were isolated and identified as inoscavin C(1),phellifuropyranone A(2),phelligridin D(3),inoscavin A(4),which had a certain inhibitory effect on the proliferation of HepG2 cells.The inhibitory effect of them on HepG2 cells increased in a dose-dependent manner within the concentration range of 5-160μmol/L.CONCLUSION All the compounds are isolated from this fungus for the first time and have certain antitumor activities.