双酚A对PK-15猪肾细胞DNA损伤和细胞凋亡的影响

Effects of Bisphenol A on DNA Damage and Apoptosis of PK-15 Porcine Renal Cells

双酚A(Bisphenol A,BPA)是一种环境内分泌干扰物,用于生产聚碳酸酯和环氧酯塑料,并被广泛用于一些日常用品中。为了探究BPA对肾脏细胞的毒性作用机制,将PK-15猪肾细胞暴露于浓度为10^(-3)、10^(-4)、10^(-5)、10^(-6) mol/L的BPA中,处理24 h后同对照组(未经BPA处理的猪肾细胞)一起进行单细胞凝胶电泳和Hoechst细胞凋亡检测。结果表明,与对照组相比,PK-15猪肾细胞彗星细胞率、彗星细胞尾长、尾部DNA百分含量和尾矩均随着BPA浓度的升高呈剂量依赖性升高(P<0.01);PK-15猪肾细胞的细胞核随着BPA浓度的升高萎缩或凝聚加剧;细胞凋亡率也呈剂量依赖性升高(P<0.01),提示BPA在PK-15猪肾细胞DNA损伤和细胞凋亡中可能起着重要作用,具有肾毒性。研究结果可为BPA对动物慢性肾病的有害影响研究奠定基础,同时为未来研究提供思路。

Bisphenol A(BPA)is an environmental endocrine interferent,which is used in polycarbonate and epoxy plastic production,as well as some daily supplies.In order to explore the mechanisms of renal toxicity of BPA,PK-15 pig renal cells were exposed to 10^(-3),10^(-4),10^(-5),10^(-6) mol/L BPA.After 24 h treatment,single cell gel electrophoresis and Hoechst cell apoptosis were performed together with the control group.The results showed that compared with the control group,the comet cell rate,comet cell tail length,tail DNA percentage and tail moment of PK-15 pig renal cells increased in a dose-dependent manner with the increase of BPA concentration(P<0.01).As the BPA concentration increasing,the nuclei of PK-15 renal cells shrank or condensed,and their apoptosis rate increased in a dose-dependent manner(P<0.01),which suggested that BPA induced cell DNA damage and apoptosis might play an important role in nephrotoxicity.These results laid a foundation for the research of harmful effects of BPA on animal chronic kidney disease,and provided research ideas for future researches.