响应面法优化油茶蒲黄酮提取工艺的研究

Study on Optimization of Extraction Process of Flavonoids from Camellia Oleifera by Response Surface Methodology

本研究以油茶蒲总黄酮提取率为指标,采用单因素试验和响应面法优化提取油茶蒲黄酮化合物的工艺。单因素试验确定的较好的因素水平为提取温度60℃、乙醇溶液体积分数70%、超声功率为90 W。响应面优化法优化结合实际操作,得到的最佳工艺条件为提取温度为60℃、乙醇体积分数65%、超声功率为72 W,得到总黄酮提取率为6.98%,与预测提取率7.08%接近,说明该响应面模型准确可靠,提高了黄酮提取率,为后续的油茶蒲中总黄酮的提取和开发提供了依据。

In this study,the extraction rate of total flavonoids from Camellia oleifera was used as an indicator,and the single factor test and response surface method were used to optimize the extraction process of flavonoids from Camellia oleifera.The better factor level determined by the single factor experiment is the extraction temperature of 60℃,the volume fraction of ethanol solution 70%,and the ultrasonic power of 90 W.Response surface optimization method optimization combined with actual operation,the best process conditions obtained are extraction temperature of 60℃,ethanol volume fraction of 65%,ultrasonic power of 72 W,and the total flavonoid extraction rate is 6.98%,which is close to the predicted extraction rate of 7.08%.It shows that the response surface model is accurate and reliable,and the extraction rate of flavonoids is improved,which provides a basis for the subsequent extraction and development of total flavonoids in Camellia oleifera.