肉苁蓉中主要活性物质的快速检测方法

Rapid Determination of Main Bioactive Compounds in Cistanches Herba

建立HPLC-DAD同时检测肉苁蓉中主要活性物质7种苯乙醇苷类成分的快速分析方法。使用Agilent ZORBAX C18色谱柱(150mm×4.6mm,5μm),以乙腈-0.1%甲酸为流动相进行梯度洗脱,二极管阵列检测器,波长330nm,外标法定量。7种苯乙醇苷类成分在检测范围内有良好的线性,相关系数均在0.9995以上,检出限为0.5-2μg/mL,定量限为2-10μg/mL。在3个水平下加标回收率为85.12%-97.21%,相对标准偏差低于5%。该方法快速、准确、简便,适用于肉苁蓉苯乙醇苷类成分的检测需求,后续可以为控制肉苁蓉质量提供有力支持和有效保障。

To establish a method for rapid determination of 7 phenylethanoid glycosides which were main bioactive compounds of Cistanches herba by HPLC-DAD.The solution was gradient eluted by a column of Agilent ZORBAX C18(150 mm×4.6 mm,5μm)with the mobile phase of acetonitrile-0.1%formic acid solution.Detection was performed using a diode array detector with 330nm detection wavelength.The quantification was performed by the external standard method.The 7 food additives showed good linearity in the range of determination,and the correlation coefficients were all above 0.9995.The limits of detection were 0.5-2μg/mL,and the limits of quantification were 2-10μg/mL.The recoveries of samples at 3 levels were 85.12%-97.21%and the relative standard deviations were less than 5%.This method is simple,rapid and accurate,which is suitable for the detection of phenylethanoid glycosides.It can provide strong support and effective guarantee for the quality control of Cistanches herba.