落新妇根药材中岩白菜素定性定量方法研究

Study on the Qualitative and Quantitative Methods of Bergenin in Astilbe Root

目的研究落新妇药材中岩白菜素定性及定量的方法。方法通过薄层色谱鉴别落新妇根药材内岩白菜素,并以高效液相色谱法对其含量进行定量分析。结果在薄层色谱别中,可见薄层图斑点清晰,整体重现性良好,在浓度测定中,选定浓度在0.025~0.250 mg/mL内线性关系良好,相关回归方程为:Y=28159x+50585,r=0.9999,回收试验结果显示回收率达97.88%(相对标准偏差为1.75%)。结论定性鉴别整体应用效果良好,具有鉴别快速、专属性较强的优点,定量分析准确度及重复性良好,在落新妇根药材质控中可取得效果,可积极推广应用于药品质控中。

Objective To study the qualitative and quantitative methods of Bergenin in Astilbe.Methods Bergenin in the root of Astilbe root was identified by thin-layer chromatography,and its content was quantitatively analyzed by high performance liquid chromatography.Results In thin-layer chromatography,and the thin-layer map spots are clear,and the overall reproducibility is good.In the concentration determination,the selected concentration has a good linear relationship within 0.025-0.250 mg/mL,and the correlation regression equation is:Y=28159x+50585,r=0.9999,the recovery test result shows that the recovery rate is 97.88%(relative standard deviation is 1.75%).Conclusion The overall application effect of qualitative identification is good.It has the advantages of rapid identification and strong specificity.It has good quantitative analysis accuracy and repeatability.It can achieve results in the control of the material of Astilbe root medicine and can be actively promoted and applied to the quality control of medicine.