复方肝毒清对急性肝损伤小鼠的护肝作用

Protective Effect of Compound Ganduqing on Acute Liver Injury in Mice

目的:探索复方肝毒清对四氯化碳(CCl_(4))引起的小鼠急性肝损伤的保护作用。方法:将60只昆明小鼠随机分为正常组、模型组、联苯双酯对照组和复方肝毒清高、中、低剂量组。除正常组外,其他各组采用腹腔注射0.4%的CCl_(4)橄榄油溶液的方法建立小鼠肝损伤的模型。不同阶段进行灌胃给药复方肝毒清,而后在实验中采用眼球摘除的方法取血,进一步获取血清。通过肝解剖进行外观观察,对血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)的活性进行检测,及对肝脏中的Fas、白细胞介素-6 (IL-6) mRNA含量进行测定,显微镜观察肝组织病理变化。结果:与正常组比较,模型组血清ALT、AST水平均上调(P<0.01),证明模型复制完成。与模型组比较,联苯双脂对照组、复方肝毒清各剂量组血清ALT、AST活性均降低(P<0.01)。与正常组比较,模型组小鼠肝脏组织Fas、IL-6 mRNA的表达上调,差异有统计学意义(P<0.01)。与模型组比较,联苯双脂对照组、复方肝毒清各剂量组肝脏Fas、IL-6 mRNA值均降低(P<0.01)。病理切片观察也表明复方肝毒清能明显改善肝损伤。结论:复方肝毒清对CCl_(4)导致的小鼠急性肝损伤有显著的保护作用,其中具体的作用机制可能与降低相关炎症因子的表达有关。

Objective:To explore the protective effect of Compound Ganduqing on acute liver injury induced by carbon tetrachloride(CCl_(4)) in mice. Methods:A total of 60 Kunming mice were randomly divided into the normal group,the model group,the positive group(biphenyl diester dripping pills) and the Compound Ganduqing groups of high-dose,medium-dose and low-dose. Except for the normal group, the mice models with liver injury in other groups were established by intraperitoneal injection of 0.4% CCl_(4) olive oil solution. Compound Ganduqing was administered intragastrically at different stages,and then the blood was extracted by the method of eyeball extirpation in the experiment to further obtain serum. The activity of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) in serum was measured,and the content of Fas and interleukin-6(IL-6) m RNA in liver tissue was determined. The pathological changes of liver tissue were observed by microscope. Results:Compared with the normal group,the levels of ALT and AST in serum were up-regulated in the model group(P<0.01),which proved that the model replication was completed. Compared with model group,the activity of ALT and AST in serum in the biphenyl diadipose group and the Compound Ganduqing groups of each dose was decreased(P<0.01). There was significant difference in the comparison of the expression of Fas and IL-6 m RNA in the liver tissue between the normal group and the model group(P<0.01). Compared with the model group,the expression of Fas and IL-6 mRNA in the liver tissue was decreased in the biphenyl diadipose group and the Compound Ganduqing groups of each dose(P<0.01)(P<0.05). Pathological observation also showed that Compound Ganduqing could significantly improve the liver injury.Conclusion:Compound Ganduqing has significant protective effect on acute liver injury induced by CCl_(4) in mice,and the specific mechanism of action may be related to the reduction of the expression of related inflammatory factors.