基于液相色谱-串联质谱的单细胞蛋白质组学方法推动肿瘤诊断研究

The single-cell proteomics methods based on liquid chromatography tandem mass spectrometry promote tumor diagnosis research

单细胞蛋白组学分析能够对细胞异质性进行深层次研究,进一步推动精准医疗和肿瘤研究等领域发展。近年来,基于质谱的蛋白质组学取得重大进展,然而在单细胞水平进行蛋白质组学分析仍然面临着灵敏度挑战。目前液相色谱-串联质谱已成为蛋白质组学主要分析方法,由于其具有高灵敏度,高通量,高稳定性等特点,在单细胞领域得以广泛推广。近几年具有代表性的单细胞蛋白质组学方法,根据样品处理方式的不同,可以分为单管技术、微流控平台、集合处理平台三类。不同的数据采集和分析方法各有优劣。单细胞蛋白质组学目前还停留在实验室研究,若能早日在临床应用,必将推动精准医疗和肿瘤学研究进步发展。

Single-cell proteome analysis can perform in-depth research on cell heterogeneity and further promote the development of precision medicine and tumor research.In recent years,mass spectrometry-based proteomics has made substantial progress,but proteomics analysis at the single-cell level still faces sensitivity challenges.At present,liquid chromatography tandem mass spectrometry has become the main analysis method of proteomics.Because of its high sensitivity,high throughput,and high stability,it has been widely used in the field of single cells.In recent years,the representative single-cell proteomics methods can be divided into three types:single-tube technology,microfluidic platform,and integrated processing platform according to different sample processing methods.Different kinds of data collection and analysis methods have their own advantages and disadvantages.If single-cell proteomics,still a laboratory study,can be applied in diagnostic practice as soon as possible,it will definitely promote the progress of precision medicine and oncology research.