丹皮炭纳米类成分的凉血止血作用研究

The effect of Moutan cortex carbonisata nano-components on the blood-cooling and hemostatic

目前国内外对于丹皮炭的研究主要集中在制炭前后化学成分的变化,缺乏与药效直接关联的物质基础研究。本研究以丹皮为前驱体,高温烧制成丹皮炭,从丹皮炭中提取分离出丹皮炭纳米类成分(Moutan cortex carbonisata nano-components,MCC-NCs),并探究其凉血止血作用。实验通过马弗炉(350℃,1 h)高温烧制丹皮炭,再从中提取分离出MCC-NCs,并利用透射电子显微镜、紫外-可见吸收光谱、荧光光谱、傅里叶变换红外光谱和X射线光电子能谱对其进行了表征;此外,该研究评估了MCC-NCs的凉血止血作用。结果显示,MCC-NCs的尺寸分布范围为0.80~2.8 nm,晶格间距为0.26 nm,主要由C、O和N元素组成,具有OH、C=O、C-N、C=C等丰富的表面官能团,荧光量子产率为7.18%。所有动物实验均符合北京中医药大学伦理委员会的规定。结果表明,预先给予MCC-NCs可显著降低全血高、中、低黏度和血浆黏度;降低红细胞压积和红细胞分布宽度,减少血红蛋白含量和红细胞计数(P<0.05);此外,MCC-NCs可以显著降低活化部分凝血活酶时间、凝血酶时间和纤维蛋白原水平(P<0.05)。病理学检查结果发现,MCC-NCs可以明显减轻肺组织损伤,减小出血和炎性细胞浸润;同时,也可以明显减轻胃黏膜出血症状。综上,实验结果显示MCC-NCs具有凉血止血的作用,其止血作用主要与内源性凝血途径或纤维蛋白原系统的激活有关,为探索中药止血的物质基础提供了新的策略。

At present,the research of Moutan cortex carbonisata(MCC)mainly focuses on the changes of chemical composition before and after charcoal production,and there is a lack of material basic research directly related to the efficacy at home and abroad.In this study,Moutan cortex,as a precursor,and was calcined to MCC at high temperature.The Moutan cortex carbonisata nano-components(MCC-NCs)were extracted and separated from MCC to explore its cooling-blood and hemostatic effects.In the experiment,the MCC was calcined at a high temperature in a muffle furnace(350℃,1 h),and then MCC-NCs were extracted for MCC,and characterized by transmission electron microscopy and UV-vis absorption spectroscopy,fluorescence spectroscopy,Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy.In addition,the study evaluated the bloodcooling and hemostatic effects of MCC-NCs.The results showed that MCC-NCs have a size distribution of 0.80-2.8 nm,a lattice spacing of 0.26 nm.MCC-NCs are mainly composed of C,O and N elements and have abundant surface functional groups such as OH,C=O,C-N and C=C.The fluorescence yield of MCC-NCs was 7.18%.The experiments complied with the Animal Ethics Committee of Beijing University of Chinese Medicine.The result indicated that pretreatment MCC-NCs can significantly(P<0.05)reduce the high,medium,and low viscosity of whole blood and plasma viscosity,and reduce hematocrit,red blood cell distribution width,hemoglobin and red blood cell level.In addition,MCC-NCs significantly reduced the levels of activated partial thromboplastin time,thrombin time and fibrinogen(P<0.05).The pathological examination results showed that MCC-NCs can significantly reduce lung tissue damage,reduce bleeding and inflammatory cell infiltration.At the same time,it can also significantly reduce the symptoms of gastric mucosal bleeding.In conclusion,the results indicated that MCC-NCs has significantly the effect of blood cooling and hemostasis,and its hemostatic effect is mainly related to the activation of endogenous coagulation pathway or fibrinogen system,which provided a novel strategy for exploring the material basis of traditional Chinese medicine for hemostasis.