基于代谢组学技术研究丹红注射液对血小板内代谢物的变化

Effect of Danhong Injection on platelet metabolites based on metabonomics technology

采用LC-MS代谢组学技术研究丹红注射液对家兔血小板内源性代谢物的影响。丹红注射液处理血小板后,测定其抗血小板聚集作用并利用代谢组学技术分析血小板内代谢物的变化。建立主成分分析(PCA)和偏最小二乘法判别分析(PLS-DA)模型进行模式识别、综合变量投影重要性(VIP)分析和独立t检验分析筛选差异代谢物,通过代谢通路初步探讨丹红注射液对血小板的潜在作用机制。结果表明不同剂量与浓度的丹红注射液对血小板聚集率起到不同的对抗作用,且随着剂量和浓度的增加,对抗作用越强。与对照组比较,丹红注射液处理血小板后共筛选出烟酸、烟酸核糖苷、次黄嘌呤等25种差异代谢物,主要涉及烟酸和烟酰胺代谢、嘌呤代谢这2条可能的代谢通路。应用代谢组学技术从微观角度去评价丹红注射液对血小板的作用,可为血小板相关机制研究提供新思路。

The effect of Danhong Injection on the endogenous metabolites of rabbit platelets was analyzed by the liquid chromatography-mass spectrometry(LC-MS)based metabonomic approach.Anti-platelet aggregation was detected after Danhong Injection treatment and the changes of platelet metabolites were analyzed by metabonomics.Principal component analysis(PCA)and partial least squares discriminant analysis(PLS-DA)were performed to investigate the effect of Danhong Injection on endogenous metabolites of platelets,characterize the biomarkers,and explore the relevant pathways and the underlying mechanism.As demonstrated by the pharmacodynamic results,Danhong Injection of different doses and concentrations antagonized platelet aggregation in a dose-and concentration-dependent manner.In contrast to the control group,25 differential metabolites such as nicotinic acid,nicotinic acid riboside,and hypoxanthine were screened out after platelets were treated by Danhong Injection.These metabolites,serving as important biomarkers,were mainly enriched in the nicotinic acid-niacinamide metabolic pathway and purine metabolic pathway.This study explored the therapeutic mechanism of Danhong Injection from a microscopic perspective by metabonomics,which is expected to provide a new idea for the investigation of platelet-related mechanisms.