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基于TCGA数据库分析肝细胞癌组织UCK2、PRIM1和DNTM1基因水平对患者预后的影响 被引量:1

UCK2,PRIM1 and DNTM1 levels in cancerous tissues of patients with hepatocellular carcinoma based on TCGA database
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摘要 目的通过分析TCGA数据库肝细胞癌(HCC)组织基因组数据,分析差异基因,寻找影响肝癌患者预后的分子标志物。方法搜索癌症基因图谱(TCGA)数据库,查找HCC组织差异基因及临床和病理学资料,进行基因筛选和生存分析。根据差异基因水平,以fdr=0.05和lgFC=1为筛选依据,绘制生存曲线,选择基因集“c2.cp.kegg.v6.2.symbols.gmt”行KEGG富集分析。结果在TCGA-LIHC数据库,收集374例HCC组织和50例癌旁组织所对应的临床和病理学参数;高风险组HCC患者总体生存率显著低于低风险组患者;研究筛选出具有显著水平性基因DNTM1、PRIM1和UCK2,进行GSEA富集分析,GSEA显示了许多显著丰富的信号通路,进一步证明了上述基因与HCC发生及与患者预后的显著性关系,从而揭示了HCC组织DNTM1、PRIM1和UCK2基因水平对生存有显著性影响。结论通过TCGA数据库筛选和验证,发现HCC患者癌组织UCK2、PRIM1和DNTM1基因水平显著上调,而CYP2C9基因显著下调,它们可以作为肝癌的分子标志物而指导临床,判断预后。 Objective To search for differential genes in cancerous tissues of patients with hepatocellular carcinoma(HCC)based on TCGA database.Methods We search the differential genes in patients with HCC from TCGA database,and draw the survival curve according to the levels of differential genes by using fdr=0.05 and lgFC=1 as the screening basis.Results We successfully got the clinical and pathological materials of 374 cancerous tissues and 50 adjacent non-cancerous tissues from the TCGA-LIHC database;the total survival in patients with high risk was significantly lower than that in patients with low risk;we found the significant higher levels DNTM1,PRIM1 and UCK2 genes to screen and verify in TCGA database,and the results showed that many abundant signaling pathway,suggesting that DNTM1,PRIM1 and UCK2 were related to the survival of patients with HCC.Conclusion The UCK2,PRIM1 and DNTM1 genes are up-regulated and CYP2C9 gene is down-regulated in liver cancerous tissues by through TCGA database screening and verification,which might help the clinicians to deal with them in clinical practice.
作者 赵艺 卢秉久 Zhao Yi;Lu Bingjiu(Liaoning University of Traditional Chinese Medicine,Shenyang 110000,Liaoning Province,China)
机构地区 辽宁中医药大学
出处 《实用肝脏病杂志》 CAS 2021年第5期729-732,共4页 Journal of Practical Hepatology
基金 国家自然科学基金重大课题(编号:2018ZX10725-505-001)。
关键词 肝细胞癌 TCGA数据库 差异基因 COX回归模型 UCK2 PRIM1 DNTM1 Hepatocellular carcinoma TCGA database Differential genes Cox regression model UCK2 PRIM1 DNTM1
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