miR-125b靶向调控Bcl-2修饰因子对生精细胞增殖的影响

Effect of miR-125b targeted regulating Bcl-2 modifier on proliferation of spermatogenic cell

目的探究miR-125b靶向调控Bcl-2修饰因子(BMF)对生精细胞增殖的影响。方法选取7日龄雄性昆明小鼠,分离得到生精细胞进行培养。将miR-NC、miR-125b mimic、miR-125b inhibitor分别转染至生精细胞中,将生精细胞分为三组。采用实时荧光定量PCR、Western blot检测各组miR-125b和BMF的表达水平,CCK-8实验检测各组生精细胞增殖能力。结果 miR-125b对BMF基因具有靶向调控作用;miR-125b mimic组miR-125b相对表达水平最高而BMF相对表达水平最低,miR-125b inhibitor组miRNA相对表达水平最低而BMF相对表达水平最高(P<0.05);miR-125b mimic组增殖能力最强而miR-125b inhibitor组增殖能力最弱(P<0.05)。结论 miR-125b对BMF基因具有靶向调控作用,miR-125b靶向抑制BMF基因表达,促进生精细胞的增殖,对男性不孕不育治疗中的精子发育过程有积极意义。

Objective To investigate effect o of miR-125 b targeted regulating Bcl-2 modifying factor(BMF) on proliferation of spermatogenic cell. Methods Spermatogenic cells were separated from 7 week old male Kunming mice for culture. miR-NC, miR-125 b mimic and miR-125 b inhibitor were respectively transfected into spermatogenic cells, and transfected cells were divided into three groups. Real-time quantitative and Western blot were used to detect the expression of miR-125 b and BMF in each group. CCK-8 assay was used to verify the proliferation of spermatogenic cells in each group. Results miR-125 b had targeted effect on BMF gene;miR-125 b mimic group had highest relative expression level of miR-125 b and lowest relative expression level of BMF. miR-125 b inhibitor group had lowest relative expression of miR-125 b and highest relative expression of BMF(P<0.05). miR-125 b mimic group had highest proliferation, and miR-125 b inhibitor group had lowest proliferation(P<0.05). Conclusions miR-125 b has targeted effect on BMF. miR-125 b accelerates proliferation of spermatogenic cells via targeted suppressing BMF, having positive significance in sperm development of male infertility treatment.