金银花对轻型新型冠状病毒肺炎治疗的药理价值

Pharmacological value of Lonicera japonica flos on the treatment of mild COVID-19 patient

目的探讨金银花提取物对轻度新型冠状病毒肺炎(COVID-19)的潜在治疗药理价值,为轻型COVID-19提供中医药防治新思路。方法利用中药系统药理学分析平台(TCMSP)筛选出金银花活性成分及作用靶点。SwissADME和pkCSM数据库预测成分药物代谢动力学特性。基因表达综合(GEO)数据库收集轻型COVID-19转录测序组数据。数据质控,DEGs鉴定及富集分析由R工具包实现。STRING和Cytoscape构建蛋白相互作用网络,利用cytoHubba插件筛选核心DEGs。运用PyMOL软件进行分子对接。结果金银花7种活性成分具有较好的口服吸收利用度和类药性特征。与轻型COVID-19相关的靶点3个:COL20A1、一氧化氮合酶2(NOS2)、双氧化酶2(DUOX2)。其共同显著富集通路5条,包括过氧化物代谢,活性氧生物合成及代谢,血红素、四吡咯结合等途径。结论金银花治疗轻型COVID-19具有多靶点多通路作用的特点。其可能作用机制是通过NOS2、DUOX2表达调控轻型COVID-19发展过程中的活性氧与血红素相关过程对这类患者进行干预。

Objective The purpose of this study was to investigate the potential pharmacological value of Lonicera japonica flos extract in treating mild COVID-19,and to provide a new idea of TCM prevention and treatment for mild COVID-19.Methods TCMSP database was used to screen out the active components and action targets of honeysuckle.SwissADME and pkCSM databases were used to predict pharmacokinetics of constituents.The GEO database collected transcriptional sequencing data for mild COVID-19.Data quality control,DEGs identification and enrichment analysis were realized by R tool.STRING and Cytoscape constructed protein interaction networks and screened core DEGs using cytoHubba.Molecular docking was performed using PyMOL software.Results The 7 active ingredients of Honeysuckle had good oral absorption and utilization and drug-like characteristics.There were three targets associated with mild COVID-19:COL20A1,NOS2,and DUOX2.There were 5 common enrichment pathways,including peroxides metabolism,reactive oxygen biosynthesis and metabolism,heme,tetrapyrrole binding and so on.Conclusion Honeysuckle had the characteristics of multi-target and multi-pathway action in the treatment of mild COVID-19. The possible mechanism of action was to intervene in mildCOVID-19 by regulating reactive oxygen species and heme related processes during the development ofmild COVID-19 through NOS2 and DUOX2 expression.