基于网络药理学探讨三七抗急性肺损伤相关分子机制

Molecular mechanism of Panax notoginseng against acute lung injury based on network pharmacology

目的:利用网络药理学的方法研究三七抗急性肺损伤的相关分子机制。方法:利用TCMSP数据库获取三七的活性成分,再通过药物动力学参数筛选出主要活性成分,并通过文献予以补充;通过Swiss Target Prediction数据库获取各活性成分靶点;通过Gene Cards、OMIM、Dis Ge NET数据库获取急性肺损伤靶点;利用STRING数据库进行蛋白质相互作用分析,采用Cytoscape3.7.2构建PPI网络并挖掘出其潜在蛋白质功能模块;采用Metascape平台对三七抗急性肺损伤靶点进行GO分类富集分析和KEGG通路富集分析。结果:共筛选出9个活性成分,槲皮素、人参皂苷Rh2和人参皂苷Rg1等可能为三七抗急性肺损伤作用的主要活性成分,核心靶点有PIK3CA、AKT1、PIK3R1、VEGFA、EGFR等。三七抗急性肺损伤作用可能涉及的通路有磷脂酰肌醇-3-激酶-蛋白激酶B(PI3K-Akt)信号通路、血管内皮生长因子(VEGF)信号通路、Ras信号通路、Rap1信号通路等。结论:本研究初步揭示了三七抗急性肺损伤的多成分、多靶点、多通路的作用机制。

Objective:To study the relevant molecular mechanism of Panax notoginseng against acute lung injury by network pharmacology.Methods:The active ingredients of Panax notoginseng were obtained by the TCMSP database,then the main active ingredients were screened through pharmacokinetic parameters and supplemented by literature.The targets of active ingredients were obtained by the Swiss Target Prediction database.The acute lung injury targets were acquired through Gene Cards,OMIM,and DisGeNET databases.The protein interaction analysis was employed by STRING database,and the Cytoscape 3.7.2 was used to construct PPI network and dig out its potential protein functional modules.The Metascape platform was used to perform GO classification enrichment analysis and KEGG pathway enrichment analysis for the anti-acute lung injury targets of Panax notoginseng.Results:A total of 9 active ingredients were screened.Quercetin,ginsenoside Rh2 and ginsenoside Rg1 may be the main active ingredients of Panax notoginseng against acute lung injury,and the core targets are PIK3CA,AKT1,PIK3R1,VEGFA,EGFR,etc.The anti-acute lung injury effect of Panax notoginseng may involve the phosphatidy linositol-3-kinase-protein kinase B(PI3K-Akt)signaling pathway,vascular endothelial growth factor(VEGF)signaling pathway,Ras signaling pathway,Rap1 signaling pathway,etc.Conclusion:This study initially reveals the multi-component,multi-target,and multi-pathway mechanism of Panax notoginseng against acute lung injury.