睡眠方颗粒剂对失眠大鼠模型的治疗作用及机制

The effects and mechanisms of Sleep Formula Granules on insomnia rats

目的观察睡眠方颗粒剂对失眠大鼠模型的治疗作用,初步探讨其作用机制。方法选取48只成年SD大鼠,分为正常组、模型组、睡眠方水丸组和睡眠方颗粒剂组。除正常组外,建立其它3组大鼠失眠模型,腹腔注射350 mg/(kg·d)对氯苯丙氨酸(PCPA)制备大鼠失眠模型成功后,睡眠方水丸组和睡眠方颗粒剂组分别给予相应的药物灌胃,模型组和正常组均给予同等体积的0.9%生理盐水灌胃。观察大鼠一般状况和体质量变化,比较各组大鼠脑组织5-羟色胺(5-HT)和多巴胺(DA)水平。结果睡眠方颗粒剂组大鼠经治疗后一般状况和体质量均得到改善,大鼠脑组织5-HT含量显著升高(P<0.05),DA含量显著降低(P<0.05);与睡眠方水丸组比较差异无统计学意义(P>0.05)。结论睡眠方颗粒剂可改善失眠大鼠模型一般状态和体质量,对大鼠脑组织5-HT和DA的调节作用可能是其治疗失眠的作用机制。

Objective To observe the therapeutic effect of Sleep Formula Granules on insomnia rats and explore its preliminary mechanisms.Methods A total of 48 SD rats were divided into normal group,model group,sleep formula pills group and sleep formula granules group.The insomnia model was established in 3 groups except for the control group.Rats were injected intraperitoneally with p-chlorophenyl alanine[PCPA,350mg/(kg·d)]to establish an insomnia model.After the success of the model,sleep formula pills group and sleep formula granules group were given corresponding drug gavage.Model group and normal group were given the same volume of 0.9%normal saline gavage.The general condition and body weight of rats were observed,5-HT and DA contents in brain tissue were compared.Results Sleep formula granules can improve the general condition and body weight of rats.The 5-HT contents in brain tissue were increased significantly(P<0.05),and the content of DA was decreased significantly(P<0.05).Compared with the Sleep formula pills group,there was no statistical?difference between the two groups(P>0.05).Conclusion Sleep formula granules can improve the general state and body weight of insomnia rats,and the regulating effect on 5-HT and DA may be the mechanism of insomnia treatment.