胃癌组织的PCED1B-AS1水平及临床意义

Level and clinical significance of PCED1B-AS1 in gastric cancer tissues

目的探讨胃癌组织的PCED1B反义RNA 1(PCED1B-AS1)水平及临床意义。方法收集本院2015年4月至2019年7月手术切除的88对胃癌组织及配对癌旁组织并采用实时定量PCR(qPCR)检测胃癌组织相较于癌旁组织的PCED1B-AS1水平,分析组织PCED1B-AS1水平与胃癌临床病理特征和预后的关系,Cox比例风险回归模型进行多因素分析。GEPIA在线分析胃癌组织PCED1B-AS1水平与程序性死亡分子1(PD-1)及其配体1(PD-L1)和PD-L2的关系。结果88例胃癌组织的PCED1B-AS1水平为3.135±0.606,高于癌旁组织的1.373±0.437,差异有统计学意义(t=22.116,P<0.001)。组织PCED1B-AS1水平与Lauren分型、TNM分期、分化程度、浸润深度和总生存期(OS)有关(P<0.05),而与年龄、性别、肿瘤直径和淋巴结转移无关(P>0.05)。PCED1B-AS1低水平组的中位OS未达,优于高水平组的45.0个月(P<0.05),进一步多因素分析发现组织PCED1B-AS1水平是OS的危险因素(OR=2.106,95%CI:1.345~3.654,P=0.012)。PCED1B-AS1水平与PD-1、PD-L1和PD-L2呈正相关(r=0.71、0.40和0.67,P<0.05)。结论胃癌组织中PCED1B-AS1的高表达与胃癌恶性程度和不良预后密切相关,可能参与了胃癌局部的免疫逃逸,有可能成为预测胃癌进展和预后的新分子标志物。

Objective To investigate the level and clinical significance of PCED1B antisense RNA 1(PCED1B-AS1)in gastric cancer tissues.Methods Eighty-eight pairs of gastric cancer tissues and adjacent tissues surgically removed in our hospital from April 2015 to July 2019 were collected,and the PCED1B-AS1 level of gastric cancer tissues in relative to adjacent tissues was detected by real-time quantitative PCR(qPCR).Relationship of tissue PCED1B-AS1 level with clinicopathological features and prognosis of gastric cancer were analyzed.Cox proportional hazards regression model was used for multivariate analysis.GEPIA online was applied to analyze the relationship of PCED1B-AS1 level with programmed death-1(PD-1)and its ligand PD-L1/PD-L2 in gastric cancer.Results The PCED1B-AS1 level in 88 cases of gastric cancer was 3.135±0.606,higher than 1.373±0.437 in adjacent tissues(t=22.116,P<0.001).The PCED1B-AS1 was related to Lauren's classification,TNM stage,degree of differentiation,depth of invasion and overall survival(OS)(P<0.05),but not to age,sex,tumor diameter and lymph node metastasis(P>0.05).The median OS in the PCED1B-AS1 low-level group was not reached,superior to 45.0 months in the high-level group(P<0.05).Further multivariate analysis found that the tissue PCED1B-AS1 level was a risk factor for OS(OR=2.106,95%CI ranging from 1.345 to 3.654,P=0.012).The PCED1B-AS1 level was positively correlated with PD-1,PD-L1 and PD-L2(r=0.71,0.40 and 0.67,P all<0.05).Conclusion The high expression of PCED1B-AS1 in gastric cancer is closely related to the degree of malignancy and poor prognosis of gastric cancer.It may be involved in the local immune escape of gastric cancer,and may become a new molecular marker to predict the progression and prognosis of gastric cancer.