柑橘大实蝇信息素鉴定及EAG测试

Electroantennographical response of Bactrocera minax to pheromones

【目的】分析和鉴定柑橘大实蝇Bactocera(Tetradacus)minax(Enderlein)信息素。【方法】采用顶空固相微萃取方法(HS-SPME)收集不同时间段柑橘大实蝇成虫挥发物,进一步结合气相色谱质谱联用技术(GC-MS)鉴定分析和触角电位技术(EAG)进行行为测试。【结果】N-(3-甲基丁基)乙酰胺为柑橘大实蝇成虫合成的一种挥发性化合物,且雌雄成虫都可以释放该化合物。触角电位技术测定不同日龄柑橘大实蝇成虫对1、10、100和1000μg 4种不同剂量N-(3-甲基丁基)乙酰胺的触角电位反应结果表明,柑橘大实蝇成虫对N-(3-甲基丁基)乙酰胺具有显著的触角电位反应,触角电位反应最适含量范围为大于100μg,剂量以及剂量×性别会导致柑橘大实蝇显著的触角电位反应差异。【结论】N-(3-甲基丁基)乙酰胺可能为柑橘大实蝇信息素,这一结果可为柑橘大实蝇化学通讯研究和生物防治应用提供一定的理论基础。

[Objectives]To analyze and identify the pheromones of Bactocera(Tetradacus)minax(Enderlein).[Methods]Volatiles from male and female adults were collected at various times by Headspace-Solid Phase Micro Extraction(HSSPME),and identified chemically by Gas Chromatography-Mass Spectrometry(GC-MS)and behaviorally by Electroantennography(EAG).[Results]GC-MS identified N-(3-methyl-butyl)acetamide as a volatile released by both adult male and female B.minax.The EAG responses of 3-day-old and 15-day-old B.minax to 1,10,100 and 1000μg of N-(3-methyl-butyl)acetamide were measured.Adults had a strong EAG response to N-(3-methyl-butyl)acetamide.The optimum concentration for EAG was from 100 to 1000μg.EAG response varied with dose and dose×sex.[Conclusion]N-(3-methyl-butyl)acetamide could be a pheromone of B.minax.The results provide information useful for further study of chemical communication in B.minax and the biological control of this pest.