北京艾蒿叶斑病菌的分子鉴定和室内毒力测定

Molecular Identification and Indoor Toxicity Test of Leaf Spot Pathogen of Artemisia Argyi in Beijing

通过对引起艾蒿叶斑病的病原菌进行分离和分子鉴定,并采用菌丝生长速率法研究了5种杀菌剂对艾蒿叶斑病菌的抑制作用。结果表明,分离出的艾蒿叶斑病菌属链格孢属(Alternaria alternata)。室内毒力实验结果表明,抑菌率最高的药剂是50%氟吡菌酰胺·肟菌酯悬浮剂和25%戊唑醇药剂。病原菌的EC_(50)值结果表明,艾蒿叶斑病菌对50%氟吡菌酰胺·肟菌酯和25%戊唑醇EC_(50)值最小,分别是0.04 mg/mL和0.28 mg/mL,说明这两种药剂对艾蒿叶斑病菌的抑菌性最强,可以作为田间药效试验研究的备选农药参考。

In this paper,the pathogen causing leaf spot of Artemisia argyi was isolated and identified,and the inhibition of five fungicides on leaf spot of Artemisia argyi was studied by mycelial growth rate method.The results showed that the pathogen was Alternaria alternata.The results of indoor toxicity test showed that the fungicides with the highest bacteriostatic rate were 50%flupirimide·oxime ester suspension and 25%tebuconazole.The EC_(50) values of the pathogen showed that the EC_(50) values of 50%flupirimide·oxime ester and 25%tebuconazole were the lowest,which were 0.04 and 0.28 mg/ml respectively,indicating that the two fungicides have the strongest antibacterial activity against the pathogen,which can be used as an alternative reference for field efficacy trials.