团头鲂scxa基因SNP位点与肌间骨数目的关联性分析

Expression of scxa and correlation analysis between SNP and number of intermuscular bones in Megalobrama amblycephala

为研究scxa基因序列的多态性与团头鲂肌间骨数量性状之间的关联性,以期进一步获得团头鲂肌间骨数目显著相关的SNP标记,通过克隆获得团头鲂scxa基因的cDNA序列,其开放阅读框为609 bp,共编码202个氨基酸。系统进化分析结果显示团头鲂scxa基因与斑马鱼、鲤、鲫、金线鲃具有较高保守性。荧光定量结果显示,该基因在团头鲂肌肉和肌间骨中的表达量均显著高于其他组织,且肌肉中的表达量显著高于肌间骨(P<0.05)。通过比较肌间骨数量极端群体scxa基因外显子序列,共获得与肌间骨数目显著相关的2个SNP位点及1个插入/缺失位点。以上结果表明,scxa基因在肌间骨发育过程中发挥潜在作用,其序列多态性与肌间骨数目具有显著相关性。

To study the association between the polymorphism of the scxa gene sequence and the intermuscular bone(IB)quantity traits,and to obtain SNPs that are significantly related to the number of IBs in Megalobrama amblycephala,the cDNA sequence of the scxa gene in M.amblycephala was obtained by cloning.Its open reading frame is 609 bp,encoding 202 amino acids.Phylogenetic analysis showed that the scxa gene of M.amblycephala is highly related with that of zebrafish,common carp,crucian carp,and golden thread carp.The fluorescence quantitative PCR results showed that the expression of scxa in the muscle and IB was significantly higher than that in other tissues,and the expression in the muscle was significantly higher than that in IB(P<0.05).By comparing the scxa exon sequence of the IB-extreme population,2 SNPs and 1 InDel that were significantly related to the number of IB were obtained.This result indicates that scxa gene plays a potential role in the development of IB,and its polymorphism is significantly correlated with the number of IB in M.amblycephala.