油藏原位微生物与储层矿物的相互作用

Interaction between in-situ oil reservoir microorganisms and minerals

以分离自冀东油田的典型油藏微生物竿属(Bacillus sp.)菌株XJ02为例,综合利用气相色谱分析(GC)、微生物分析、pH值、氧化还原电位(E h)、电导率(σ)、矿物质量变化、X射线衍射(XRD)和扫描电镜能谱分析(SEM-EDS)等方法,结合计算软件PHREEQC,探索微生物与白云石、云母和方解石相互作用情况。结果表明:菌株XJ02可以增大矿物的溶解度,增溶程度受矿物种类和矿物颗粒粒径的影响,矿物种类影响顺序为云母>方解石>白云石,矿物颗粒粒径影响顺序为0.074 mm方解石>0.900 mm方解石;矿物促进了菌株XJ02的生长,其影响顺序为云母>0.074 mm方解石>0.900 mm方解石>白云石;XJ02的代谢活动及其对环境理化性质的改变促进了矿物的溶解,而矿物的溶解又有效地促进了菌株XJ02的生长。

Taking typical reservoir microorganism Bacillus sp.strain XJ02 as an example,the interaction between microbes and three minerals(dolomite,calcite,and mica)of oil reservoir was investigated.The interaction process was characterized by gas chromatography(GC),microbial analysis,pH value,redox potential(E h),conductivity(σ),mineral quality,XRD and SEM-EDS,as well as the calculation software PHREEQC.The results show that XJ02 could increase the solubility of minerals,and the degree of solubilization is affected by mineral species(mica>calcite>quartz)and mineral particle size(0.074 mm>0.900 mm).Minerals promotes the growth of XJ02(mica>0.074 mm calcite>0.900 mm calcite>dolomite).The metabolic activities of XJ02 and its changes in environmental physical and chemical properties enhance the dissolution of minerals,which in turn effectively stimulates the growth of XJ02.