LncRNA44372在卵巢癌中的差异表达及生物信息学分析

The differential expression and bioinformatics analysis of lncRNA44372 in ovarian cancer

目的:探讨lncRNA44372在卵巢癌中的表达及其在卵巢癌发生发展过程中的作用机制。方法:从NCBI GEO数据库中下载卵巢癌样本及其对照样本的基因芯片数据GSE119054,选用在线工具CRN分析在卵巢癌中差异表达的lncRNA;利用在线工具Annolnc筛选与lncRNA44372有关的共表达基因,然后使用David进行基因本体论(Gene Ontology,GO)分析、信号通路富集分析;通过starbase构建lncRNA44372可能存在的竞争性内源RNA网络;用qRT-PCR检测lncRNA44372在卵巢癌细胞系中的表达。结果:GSE119054数据集中筛选出在卵巢癌中表达上调的lncRNA有6个,表达下调的lncRNA有4个;GO分析显示,与lncRNA44372相关的共表达基因在细胞凋亡、细胞周期调控和蛋白苏氨酸/酪氨酸磷酸酶活性中具有调控作用。信号通路分析显示,与lncRNA44372相关的共表达基因主要参与细胞凋亡、肿瘤形成及发展相关的信号通路。lncRNA44372可与miR-190和miR-193结合并调控Unc-51自噬激活激酶-2(ULK2)和依赖LON的过氧化物酶体-2(LONP2)在卵巢癌中的表达。qRT-PCR结果显示,lncRNA44372在卵巢癌细胞系中表达量较卵巢正常细胞系低(P<0.05)。结论:lncRNA44372在卵巢癌中呈低表达,其可能通过影响细胞凋亡、细胞周期等参与卵巢癌的发生发展。

Objective:To explore the expression of lncRNA44372 and its potential mechanism in the development of ovarian cancer.Methods:The gene chip data GSE119054 of ovarian cancer samples and their control samples was extracted and downloaded from the NCBI GEO database,and the differential expressions of lncRNA in ovarian cancer were analyzed by using the CRN online tool.Annolnc was used to pick out the co-expression genes related to lncRNA44372.Gene Ontology and signal pathway enrichment analysis were performed to draw the outline of the possible biological function of lncRNA44372 in ovarian cancer by the online software David.The divinable ceRNA network of lncRNA44372 was constructed by the starbase,an online analysis tool.The expression of lncRNA44372 was detected by qRT-PCR in ovarian cancer cell lines.Results:In the GSE119054 data set,there were 6 up-regulated lncRNA and 4 down-regulated lncRNA in ovarian cancer.The related co-expressed genes of lncRNA44372 were enriched in some biological processes,such as cell apoptosis,cell cycle regulation,protein threonine/tyrosine phosphatase activity.The related co-expressed genes of lncRNA44372 were mostly involved in signaling pathways about cell apoptosis and tumor pathogenesis and progression.LncRNA44372 may act as a microRNA sponge to bind with miR-190 and miR-193 and regulate the expression of ULK2 and LONP2 genes in ovarian cancer.qRT-PCR showed that the expression of lncRNA44372 in ovarian cancer cells was lower than that in normal ovarian cells(P<0.05).Conclusion:LncRNA44372 was lowly expressed in ovarian cancer and it may play a role in the development and progression of ovarian cancer by influencing cell apoptosis,cell cycle and so on.